Effects Of Bone Marrow Stem Cell Mobilization On Renal Interstitial Capillary And Fibrosis In Unilateral Ureteral Obstruction Rats

Objective: To investigate the mobilization effects of bone marrow cells and endothelial progenitor cells by combination of stem cell factor(SCF) with granulocyte colony-stimulating factor (G-CSF) in unilateral ureteral obstruction rats, the homing effects of bone marrow stem cells, and their effects and possible mechanisms of bone marrow stem cell mobilization on renal peritubular capillary and fibrosis. It was possible for stem cell transplantation to delay the chronic progression of renal diseases.Methods: 56 healthy male Wistar rats were divided into seven groups. ①Control group was injected normal saline 2ml/(kg·d) subcutaneouly daily for five days. ②SCF group was injected SCF 200μg/(kg·d) subcutaneouly daily for five days. ③G-CSF group was injected G-CSF 200μg/(kg·d) subcutaneous daily for five days. ④SCF-G group was injected SCF 200μg/(kg·d) and G-CSF 200μg/(kg·d) subcutaneous daily for five days. ⑤Sham group was operated by sham surgery, and then it was injected normal saline 2ml/(kg·d) subcutaneouly daily for five days. ⑥UU0 group was operated by ligation of right ureter, and then it was injected normal saline 2ml/(kg·d) subcutaneouly daily for five days. ⑦UUO+SCF-G group was operated by ligation of right ureter, and then it was injected SCF 200μg/(kg·d) and G-CSF 200μg/(kg·d)...