Treatment Of Rabbit Femoral Head Necrosis Model By Tissue Engineering Bone: An Experimental Study

[ Objective ] We made tissue engineering bone (BCBB /BMP/bFGF/MSCs) and femoral head necrosis(FHN) model of rabbits induced by microwave heating, and transplanted this tissue engineering bone to repair the rabbit FHN for evaluating the effect of this tissue engineering bone on the treament of the FHN in angiogenesis and osteogenesis and the feasibility applied in clinic.[Methods] The current study was conducted in four parts.In part one, developing a femoral head necrosis model of rabbits induced by microwave heating. We inserted the microwave antenna into the rabbit femoral head. 96 femoral heads in 48 rabbits were randomly divided into four groups basis on the microwave temperatue and heating time. There were twenty four femoral heads in every group. Group A was heated in 10 minutes by 50℃ microwave. Group B was heated in 10 minutes by 55℃. Group C was heated in 20 minutes by 55℃ and group D was heated in 10 minutes by 60℃. The rabbits were killed respectively immediately, in the 2nd, 4th, 8th and 12th weeks after operation, and then the femoral heads of each rabbit were taken off at each interval. Four femoral heads were harvested at every time point in one group. A series of examinations were carried out including of gross observation, X-ray, histology and MRI to observe the femoral head necrosis and repair, and to decide optimum microwave temperatue and heating time.In part two, the preparing of biphasic ceramic biologic bone (BCBB). The swine vertebral cancellous bone with cortex on one side was calcined to make true bone...