Protection And Repair Of RhGH In Ischemic Reperfusion Injury Of Rat Liver

Objective: To investigate the protection and repair of rhGH in ischemic reperfusion injury of rat liver and its mechanism.Methods: Part I One hundred and ten male Sprague Dawley(SD)rats, which weight 200-300g , were randomly divided into three groups: rhGH group(n=50) , controlgroup (n=50) and blank control group (n=10). In rhGH group, the rhGH was administered to rats via hypodermic injection (0.2UI/100g SD rats weight, oncedaily )seven days before establishment of the model of hepatic ischemic reperfusion,in control group, rats were treted as rhGH group except rhGH was replaced by normal sodium.then, the rhGH group and the control group were further divided into 5 groups equally according to the period of ischemic reperfusion, as 45 minutes , 1 hour, 2 hours, 4 hours, 6hours following establishment of the model, the level ofEC( Energy charge), concentrations of MDA(malondialdehyde), IL-1a(interleuin-1a),TNF-a (tumor necrosis factor-a) , ALT (alanine aminotransferase) , AST(aspartate aminotransferase ) values in serum were detected. Hepatic tissue was sectioned for immunohistochemical staining to detect the expression of NF-κB (nuclear factor kappa B) and for in situ hybridization staining to detect the expression of ICAM-1(intercellular adhesion molecule-1) mRNA on SEC (sinusoidal endothelial cells).Also, histopathological were determined. Part II Ninety Male Sprague Dawley( SD ) rats , which weight 200-300g , were randomly divided into:rhGHgroup(n=40) , control group (n=40) and blank control group(n=10). In rhGH group,the rhGH was administered to rats via hypodermic injection (0.2UI/100g SD rats weight, once daily ) seven days after establishment of the model of hepatic ischemic...