| ObjectiveIn order to study the pathophysiological mechanism of cognitive disfunction in patient with obstructive sleep apnea hypopnea syndrome (OSAHS) , with intermittent hypoxia rat models, we observed the effect of intermittent hypoxia on apoptosis of neuronal cells in rats, evaluated the expression of iNOS and HIF -1α in the brain of rats and their effects on apoptosis of neuronal cells, and measured HIF -1 transcription activity which maybe related to the molecular mechanism of hypoxia respond reaction of neural system.Methods1 Materials24 male mature wistar rats, 300 - 350g weight, mixed gas, gas exchange control system, oxygen concentration monitoring device; antibody of iNOS and HIF-1α; Nuclear/Cytosol Fractionation kit;Trizol solution;ultraviolet light instrument; PCR cycler;One Step RNA PCR Kit;γ-P32 labeled dATP et al.2 Experiment proceduresMale wistar rats were randomly divided into three groups: chronic hypoxia group (n =8, breathing 10% O2 for 8 hours/day) , intermittent hypoxia group (n = 8, breathing 10% O2 and air which were shifted per 90 second for 8 hours/ day) and control group (n= 8, breathing air). Experiment was done from 8: 00Am to 4:00Pm everyday (almost sleep time of rats) , 30 days later all animals were killed.3 Measurement methods...
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