The Effect Of Flow Shear Stress On The Expression Of Ca Ⅱ And Cat K In Osteoclast

In the stomatology realm, stress and strain exerts effect on the stomatognathic system under complicated and precise regulation, many physiological and pathological processes, such as tooth eruption, Orthodontic movement of tooth, implantitis, periodontitis, are related closely with the mechanic influence. The local change of bone tissue under the mechanical stimulation maintains and remodels the structure of bone to adapt the continuous change of the mechanical environment. Physiological loads not only produce gradient of stretch, but also the localized gradient of pressure which induct the interstitial fluid shear stress. The local fluid shear stress play an important role on the remodeling of bone. The loading reaction of bone cells and the mechanism of mechanical signal transduction are the important content to study the mechanism of bone remodelling, many studies are focusing on the mechanical research of bone cells in stomatology and orthopaedics.Osteoclasts are terminally differentiated cells, the cultivation and purification of osteoclasts are more difficult than osteoblasts and osteocytes. It's essential for the research of molecular biology to yield riched and purified osteoclasts in vitro, so the development of cultivation and purification techniques of osteoclasts are very important. The effect of rat age on the culture of osteoclasts and the purification of bone marrow-derived rat osteoclasts are rarely reported. The effect of fluid shear stress on the expression of Ca II and Cat K, the...