An Experimental Study Of Establishment Of Brain Tumor Animal Model And Gene Therapy For Glioma With Ectopic P21～(WAFI/CIP1) Gene

Glioma is one of the most common malignant tumors in the central nervous system. Since there has not been a simple and effective glioma animal model, research on the mechanism of occurrence and development of glioma has been seriously affected. In management of glioma, despit of comprehensive adoption of measures of operation, chemotherapy, radiotherapy and even biotherapy, low cure rate, high death rate, and high recurrence and disabling rate remain. Therefore, establishment of an ideal glioma animal model and discovery of an effective cure for glioma are of utmost significance to study of the tumor.This paper, based on the previous studies, is intended to establish a chemical agent induced glioma model and a syngeneic transplantation glioma model using N-nitrosoethylurea and the rat's C₆ glioma cells, and to study expression of suppressor gene P21^WAF1/CIP1 in tissues of glioma and its effects on the growth of the tumor cells so as to provide a theoretical basis for clinical application of P21^WAF1/CIP1 to management of glioma.Part I Establishment of brain tumor animal model1. The Sprague-Dauley rats were inoculated with a single dose of 50 or 70mg/kg of N-Nitrosiethylurea(NNEU) on the 18-20th days of gestation. Tumors developed in the nervous system of about 86.49%(32/37) of...