Biological Effects Of PGE₂, TNF-α And IL-1β On Human Dental Pulpal And Periodontal Fibroblast And Osteoclastic Formation In Vitro And PGs Contents In Dental Pulps

Recent advances in molecular biology and immunology have led to the new concept that cytokines and PGs have important modulation in inflammation ProstaglandinE₂(PGE₂) /Tumor necrosis factor-α(TNF-α) and Interleukin-1β(IL-1β) are the important inflammatory mediators. The paper studied the effects of three agents on the growth,DNA synthesis and ultrastracture of human dental pulpal(HDP) cell, periodontal ligament(PDL) cell, Ca metabolism of rat calvaria, osteoclast formation and its bone resorptive activity in a long-term human bone marrow culture and PGs contents in human dental pulp.1.The culture and characteration of HDP and PDL cells in vitro.HDP and PDL cells were cultured from the explants of huamn dental pulp and periodontal ligament connective tissue of the teeth extracted for orthodontic needs from the same individual, Immunohistochimicai stain and morphological examination demonstrated that the cultured cells were originated from mesenchymal. 5HDP(from 38 teeth) and 8PDL(from 40 teeth) cells were subcultured successfully and the cells displayed some stable growth characteristics within 5-10th passages.The doubling times of HDP cells and PDL cells were about 45.6hs and 27.2hs , respectively,on passage5.2.The effects of three agents on the growth and DNA synthesis of the cells.The effects on the growth and the DNA synthesis were tested by MTT assay and ³H-TdR incooperation.HDP cells and PDL cells were exposed to PGE₂,TNF-α , IL-1β and their various binds for 1 —3 days.The results showed the following:...