Cloning Of Apoptosis Related Genes In Leukemia Cell Line HL-60 Using CDNA Representational Difference Analysis

It goes without saying that apoptosis is a very important field in tumor research. Recent studies revealed many molecular mechanisms and regulation . details of apoptosis, such as the enlightenment of the role of caspases, mitochondria and bcl-2 family, which are important molecules in signal transduction pathway or regulation of apoptosis. However, much remains to be learned about the precise mechanism of apoptosis, its regulation by multiple apoptosis related genes, and etc.cDNA representational difference analysis (cDNA RDA) is a well-developed technique used to analyze differential expression. Compared to other differential analysis method like traditional subtractive hybridization and differential display PCR (DD-PCR), it has a number of significant advantages: effectiveness, sensitiveness, specificity, reproducibility, and etc. Since it appears, it has become an effective approach of gene cloning. The basic principle of RDA is based on the generation of cDNA amplicon known as representations of original cDNA, by...