A Study Of Functional Sites Of General Anesthesia And Its Electrophysiology In Central Nervous System

The mechanism of general anesthesia has been studied for about 150 years. But its functional sites has not been clarified so far, primarily due to the fact that no method has been found fit to display the mysterious state of general anesthesia in the central nervous system. In the past few years, quite a number of papers have suggested that activation of immediate early genes (IEG, Proto-oncogenes) such as c-fos oncogene, called the third messenger, participate in message transmission in the central neuron action. When the central neuron was stimulated , the expression of fos protein increased rapidly in the related neurones which can be detected by immunohistochemical technique .The present paper aims at: (1) Locating the functional site of general anesthesia in central nervous system with immunohistochemical single-labeled method;(2)Detectmining the related neurotransmitters with immunohistochemical double labeled method; (3)Studying the effects of general anesthesia on the spontantaneous neural discharge and Cl^-1 chennel of functional neurones from rat hypothalamic slices with whole cell patch clamp.RESULTS 1.Fos protein expressionThe Fos-positive neuron induced by enflurane, isoflurane, methaxyflurane and halothane anesthesia were mainly situated in the telencephalon, diencephalon and brain stem. They appeared mostly in telencephalon, including amygdaloid nuclear complex, accumbens nucleus(ABC), laterodorsal thalamic nucleus(LD), lateral septal nucleus(LS)), bed nucleus of the stlia terminal is(BST), piliform cortex(Pir), field CAl of Ammon's horn (CAl) and islands of calleja(Icj) ; in diencephalon, including habenular nucleus(Hb),...