Differentially Expressed Proteomics During Carcinogenesis And Progression Of Human Hepatocellular Carcinoma

Hepatocellular carcinoma (HCC), of which the morbidity and mortality are in the forefront among the overall tumors worldwide, is one of the malignancies with difficult in early diagnosis and extremely poor prognosis. The formation of HCC is a multistep, multistage and multifactor process like most other tumors. The conventional strategy of research on single gene has been challenged by the rapidly progress of proteomics, which can be utilized to observe the dynamical changes of nature and quantity of total protein during progression of disease. Proteomics can be used to find new protein markers and key molecules and therefore is an effective and high-throughout method for exploring mechanism of disease and for searching targets of diagnosis or therapy. By optimizing the technical platform of two-dimensional electrophoresis (2-DE), in the present study we observed dynamically the differentially expressed proteins of liver tissue during carcinogenesis and progression of human Hepatocellular Carcinoma,which including normal liver tissue, chronic type B hepatitis tissue, cirrhotic tissues, HCC tissue.We got a group of significant differential proteins, and ANXA4, Prxâ…¡,Prxâ…¢proteins which may be related to hepatocarcinogenesis was further confirmed by Western blot and RT-PCR. We also drew a positive conclusion through further analysis and validation of the biological function of ANXA4 with RNA interference technique and some following experiments related to cell properties of carcinogenesis. The entire study included four parts as following.