The Serial Research On Neuroprotective Effect Of ShenFu Injection On Cerebral Ischemia Reperfusion Injury

Part I: The setting up of intraluminal thread approach in making focal cerebral ischemia reperfusion model in rabbit and evaluation of image featuresObjective: To establish a stable model of intraluminal thread approach in making focal cerebral ischemia reperfusion model in rabbit and to evaluate it with magnetic resonance imaging (MRI) and neurological score.Methods: Forty white New Zealand rabbits were randomly divided into three groups:A,B,and C.Angiography of internal carotid artery(ICA) was performed in Group A(n=5). Control Group B(n=5) was sham-operated.In Group C(n=30),the focal cerebral ischemia reperfusion model was made by inserting a wire(d=0.45mm) into middle cerebral artery(MCA) through ICA and common carotid artery(CCA) directly and pulling it out 3 hours later. MRI and neurological deficit score were used to evaluate the effectiveness of models.Results: Angiography can show the course and bifurcation of ICA clearly.22 models were successful.The pathological changes were shown on MRI.The neurological deficit score (reperfusion 1h) was 2.8±0.45.Conclusion: The focal cerebral ischemia reperfusion model made by inserting a wire(d=0.45mm) into MCA through ICA and CCA directly and pulling it out 3 hours later was stable and fit for imaging research.Part II: The serial MRI after rabbit focal cerebral ischemia reperfusion injury and its correlation with pathological changesObjective: To explore the serial MRI after rabbit focal cerebral ischemia reperfusion injury and correlate it with the pathological changes.Methods: 27 white New Zealand rabbits were randomly divided into two groups:A and B.Control Group A(n=5) was sham-operated.In Group...