The Influence Of The Exogenous IL-18 Gene On The Biological Characteristics And The Chemosensitivity Of Rat C6 Glioma Cells

The researches have manifested that the amplification of oncogene and the inactivation of anti-oncogene or abnormal expression of cell cycle related gene are involved in the occurrence, progress, the malignant degree and prognosis of the glioma. With the advance of molecular biological research in recent years, some progress in the glioma gene therapy have been obtained, some of them has already turned into the clinical trial stage or application in the clinic, providing a new path for the gene therapy for the glioma. Cytokines are small molecule dissoluble protein with various biological activities. They act on the cells themself or other cells in autocrine or paracrine manner. They are mainly generated by lymphocyte and mononuclear macrophage, some kind of tumor cells can also secrete some cytokines.The cytokine disturbance is one of the important reasons that the immune system fails to kill tumor cells effectively. Interleukin-18(IL-18) is a cytokine found recently, having various biology activities. It can activate T cells and NK cells, make the Th1and NK cells generate INF-γ, possessing anti-tumor activity in vivo and in vitro in the presence of T cells or NK cells. However,there is a research report that the IL-18 can induces the apoptosis to tumor cells in vitro in the absence of the T cells and NK cells;after neutralization of the role of NK cells or INF-γwith antibody against NK cells or INF-γ, IL-18 still possess anti- tumor activity in vivo, but their accurate mechanism remains unknown. For this reason, the purpose of this research is to investigate the influence of the exogenous IL-18 gene on the biological characteristics and the chemosensitivity of IL-18 gene transfected-C6 glioma cells in vitro. This study includes following four parts.