Studies On Mechanisms Of Autologus Hematopoietic Stem Cell Implant Purification Using Electromagnetic Radiation

Autologus hemetopoietic stem cell transplantation is an important new technologic method to cure malignant blood tumor , substance tumour as well as some heredity diseases . At present, as for the autologus hemetopoietic stem cell transplantation for leukemia, further purification was not adopted after blood cells were collected. How to make the stem cells purified effectively, becomes a key technology to lengthen survival time of patients. In these studies, molecular biology and cytobiology methods were used to research the mechanisms of the purification effect of electromagnetic irradiation.There are seven chapters in these studies, contents of each chapter are as below:In Chapter One, the progresses of autologus hemetopoietic stem cell transplantation researches and electromagnetic biological effect researches were summarized;In Chapter Two, some methods such as electron microscope, DNA ending label and MTT measurement were used to studied the changes of apoptosis , necroses and cell vigour of leukaemia K562 cell trunk and HL-60 cell trunk irradiated with millimeter wave. Different effects of millimeter wave to leukaemia cell trunks and human marrow cells were observed clearly. Human marrow cells were not so sensitive to millimeter wave radiation as leukaemia cells.In Chapter Three, the FCA method was used to studied the changes of calcium ion consistency and the content of NO inside the leukaemia cells after irradiated with millimeter wave. It was found that millimeter wave radiation may harm leukemia cells by regulating calcium ion consistency and NO inside the cells.In Chapter Four, the changes of free radical and antioxidase in HL-60 cells after irradiated with millimeter wave were studied. It was discovered that...