The Study Of Recombinant Human Granulocyte Colony-Stimulating Factor Mobilized Peripheral Blood Mononuclear Cells To Be Cultured For Endothelial Progenitor Cell And To Therapy Diabetic Foot Disorders

Objective:1 The aim of this study is to establish the method for in vitro culture of adult human peripheral blood mononuclear cells (PBMNCs)-derived endothelial progenitor cells (EPCs) and to investigate their biological characteristics.2 To assess the efficacy of autologous transplantation of recombinant human granulocyte colony - stimulating factor (rhG-CSF) -mobilized PBMNCs in the treatment of critical Diabetic Foot Disorders and to evaluate the effect of this novel therapeutic approach on diabetic peripheral neuropathy.3 To investigate the change of the plasma level of vessel endothelium growth factor ( VEGF ) and transforming growth factor-β₁ (TGF-β₁) in patients with diabetic foot therapied by autologous transplantation of rhG-CSF mobilized PBMNCs.Methods:1 Human buffy-coat obtained using Ficoll separation from rhG-CSF mobilized PBMNCs had plated on FN-coated plates using...