Study On The Effect Of Bone Marrow Stromal Cells And Adhesion Molecules To The Growth Of Myeloma Cells

Multiple myeloma (MM) is a neoplasm characterized by accumulated malignant plasma cells which can secret a large mount of monoclonal immunoglobulin in bone marrow. In addition, almost all of myeloma cells are located in marrow cavity, hardly spreading to peripheral blood or other organs. Many investigators studied the mechanism of cell residency in bone marrow, and bone marrow microenvironment was thought to support the growth of myeloma cells and thus to play an important role on the pathogenesis of MM, especially through many adhesion molecules and cytokines. Nevertheless, the pathogenesis of MM is not completely clear yet. Most of previous studies applied myeloma cell lines as objects. In order to mimic the in vivo marrow environment, we established the co-culture system of freshly separated myeloma cells and bone marrow stromal cells (BMSCs), using myeloma cell line as control at the same time. We also investigated the effect of normal and MM BMSCs on the growth of myeloma cells and the role of four adhesion molecules. The relationship between BMSCs and myeloma cells was discussed in detail in this article.Firstly, a flow cytometry method of CD45/side scatter (SSC) gating...