Study On The Relationship Between β-haemolytic Streptococci, Mycobacterium Tuberculosis And Psoriasis

Psoriasis is a worldwide disease. Many researchers are exploring its etiology. We collected 36 guttate psoriasis and plaque psoriasis, 30 psoriatic erythroderma and pustular psoriasis, 15 psoriasis arthritis to study on the relationship between β -haemolytic Streptococci, Mycobacterium tuberculosis and psoriasis. This dissertation is divided into 3 chapters to present our research reports. The first chapter displays the study on the relationship between β -Streptococcus pyogenes and psoriasis. The second chapter elucidates the results of the study on the relationship between Mycobacterium tuberculosis and psoriasis. The third chapter analyses the components of protein antigen from β -haemolytic Streptococci and Mycobacterium tuberculosis which caused immunologic reaction in sera from patients with psoriasisLaboratory technique:1. To establish the methods of preparing whole β-haemolytic Streptococci, Mycobacterium tuberculosis and their supernatant culture extracts, cytoplasmic extracts as antigens;2. To make the plates for ELISA about whole β -haemolytic Streptococci, Mycobacterium tuberculosis and their supernatant culture extracts, cytoplasmic extracts;3. To optimize ELISA for detecting the antibody against whole β -haemolytic Streptococci, Mycobacterium tuberculosis and their supernatant culture extracts, cytoplasmic extracts;4. To introduce the sensitive methods of silver staining for displaying the peptide spectrum of SDS-PAGE and supersignal West Pico chemiluminescent substrate for displaying positive bands Western-blotting.