Cloning And Characterization Of Oxidized Low Density Lipoprotein Responsive Genes In Human Vascular Endothelial Cells

Atherosclerosis (AS) is one of the major diseases threatening human health, and is the basic pathogenesis of myocardial and cerebral infarctions and ischmia. AS is characterized by accumulation of intra- and extracellular lipids, monocytes /macrophages infiltration, foam cell formation, proliferation of arterial smooth muscle cells and accumulation of connective tissue components. LDL is associated with the development of vascular lesions and, oxidized LDL (Ox-LDL) is a key pathogenetic derivative of native LDL that may be involved in many of the above mentioned features of atherogenesis.Endothelial injury is the initiative event to atherogenesis. Ox-LDL may activate endothelial cells by inducing expression of cellular adhesion molecular, stimulating endothelial secretion of momocyte chemoattractant protein-1, recruitment of the periphery monocytes. Adherent monocyte can transmigrate into intima, transform into macrophages, and take up Ox-LDL by the scavenger receptor through the non-inhibition pathway. The study of structure and function of genes related to endothelial activation /dysfunction induced by Ox-LDL will improve our understanding to the molecular mechanism of atherogenesis.The differential display reverse transcription polymerase chain reaction (DDRT-PCR) technique was applied to compare mRNA from Ox-LDL activated human umbilical vein endothelial cells (HUVEC) and control HUVECs, 87 differential displayed cDNA fragments were obtained. 35 of the 87 cDNA fragments were chosen to be cloned and sequenced. The differential expression pattern of the...