| It has long been considered that intraocular hypertension is the major risk factor for glaucomatous optic neuropathy; retina and optic nerve are target organs of glaucoma damage. Pathological mechanism of glaucomatous optic neuropathy is progressive death of retina ganglion cells (RGCs) and optic nerve fiber loss, which leads to irreversible optic function damage. related genes and factors participate in glaucomatous occurrence and development.Objective: The purpose of this study is to search and find these unknown genes, so the mechanism of glaucomatous retina and optic nerve damage can be deeply understood on molecular level, which can provide significant basis for glaucomatous treatment and prevention.Method: Oligo microarray technique is an advanced molecular biological platform which can profile the dynamic changes of more than ten thousands of genes. In this study, a total of 6 RNA samples from different time points(7,35,60,90,180 and 360d) after laser operation were screened by large-scale Oligo microarray of 35,000 points to identify genes related to retina injury under chronic high IOP. We divided our paper into 3 parts:The first part: The foundation of chronic high IOP animal modelSD rats were used to be animal models,the left eyes were taken to be injury models, while the lateral eyes were taken as controls. Established rat model with chronic elevation of IOP and optic neuropathy changes. Using a diode laser with wavelenth of 532-nm burned at the trabecular meshwork and episcler veins at 80 SD rats,while no factor to the control. Tono-penn XL Tonometer was used to measure IOP to gurantee IOP value at 7, 35, 60, 90,180 and 360 day after operation under Pentobarbital Sodium anesthesia, the F-VEP were monitored at the model of rats after laser burned 60 and 180 days later. The retina and optic nerve samples were obtained for morphological observation to judge whether the model... |
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