A Study On The Transcriptional Regulation Mechanisms Of Human Dentin Matrix Protein 1 Gene

The dentinogenesis and mineralization are the most important parts of the tooth development and dental pulp repair after injury. Dentin non-collagenous proteins (NCPs) play critical roles in odontoblast induction, differentiation and dentin mineralization, and they have become the research focus of the tooth developmental biology and dental pulp biology. The present, most studies in this field mainly focus on dentin dentin-specific proteins: dentin sialophosphoprotein (DSPP), dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). Dentin matrix proteinl (DMP1) is one important member of the mineralization tissue-specific proteins in dentin matrix, and few reports about its molecule mechanisms during dentinogenesis and dental pulp repair after injury are available. Questions like how the odontoblasts synthesize and secrete DMP1, which factors are involved in the transcriptional process of DMP1 and what interreactions between those transcription factors remain unanswered.DMP1 is a mineralized tissue matrix protein synthesized by osteoblasts, hypertrophic chondrocytes and ameloblasts, as well as odontoblasts. It is rich of serine and its genetic structure is similar in different species and highly homologous in some highly conservative regions. It is believed to have multiple functions in vivo, acting both as a signaling molecule and a regulator of biomineralization. It acted as an HA nucleator, increasing the amount of mineral nodoules by assemblying the collagen fibrils and binding calcium ions and initiating mineral deposition. Overexpression of DMP1 in pluripotent and mesenchymal derived cells can induce these cells to differentiate and form...