Combined Somatostatin Receptor â…¡ Gene With Somatostatin Analogue Inhibit Growth Of Human Pancreatic Cancinoma

Purpose:To observe whether it can inhibit the growth of pancreatic carcinoma cells in vitro and vivo experiments with NC-8-12(specific agonist of somatostatin receptor II) and octreotide by transfecting pancreatic carcinoma cells and pancreatic carcinoma hypodermic model of naked rat with a recombinant adenovirus vector ,named as Adeno-X-SSTR2,which takes SSTR2 gene.Methods: SSTR2 gene was cloned and recombined into Adeno-X-SSTR2 gene,to observe the inhibition of NC-8-12 and octreotide of various dose (10-8M,10-9M) to pancreatic carcinoma cells of transfected and untransfected SSTR2 gene by infecting MIA-PaCa-2,AsPC-1 separately in vitro by amplification and purification technique;constitute matastasis models in vitro,and observe the influence of NC-8-12 to matastasis ability of pancreatic carcinoma cells infected with SSTR2 gene;inspect the variety of Survivin expression influenced by virous concentration NC-8-12 and octrotide by using RT-PCR semi-quantitative method and immunocytochemistry;constitute hypodermic model of naked rats with infecting and uninfecting SSTR2 gene separately,which were given NC-8-12 and octreotide of various dose(50,100,200ug/kg/d) by hypodermic injection for 3 weeks,and then killed the rats 1 weeks later.to observe the change of tumor volume and weight by taking normol saline group as control group.inspecting the expression of SSTR2 in tumor tissue by immunohistochemistry technique. Results: Adeno-X-SSTR2,recombinant adenovirus vector,can infect pancreatic carcinoma cells MIA-PaCa-2 and AsPC-1 efficiently,which both can express SSTR2 mRNA and their protein. NC-8-12 and octreotide have no inhibition to pancreatic carcinoma cells with untransfecting SSTR2 gene in vitro experiment (P>0.05). NC-8-12 and octreotide can inhibit tumor cells proliferation induced by insuline signifiently at the dose of 10-8 M (P<0.05). NC-8-12 has no inhibition to transfer procession of transfecting SSTR2 gene pancreatic carcinoma cells in vitro matastasis models(P>0.05). Survivin expression of...