Expressional Changes Of CLC-2, CLC-3 Chloride Channel In Hippocampus Of Rats Following Induced Epilepsy By Lithium-Pilocarpine

Objective To investigate the distributional character of voltage-gated chloride channel clc-2, clc-3 in rat's hippocampal formation, and its changes in chronic epileptic model induced by lithium-pilocarpine. To discuss the significance of these changes, then, to illuminate the role of chloride channel in epileptic pathogenesis.Motheds 60 Wistar rats used to make epileptic model by lithium and pilocarpine ip., and observed the rats' behavior changes. At three different times ( 24h, 14 days and 30 days ) after seizure, 30 were fixed by perfusion with 4% formamint, the brains were took out for immunohistochemistry. We observed the distributional character of voltage-gated chloride channel clc-2, clc-3 in rat's hippocampal formation and the optical density changes of which at different time after seizure. Other 30 were broken head quikly and removed hippocampus for RT-PCR, then we observed the changes of clc-2, clc-3 mRNA at different time in hippocampus after seizure.Results According to epileptic animal's behavior and EEG, the epilepsy can be divided as : the acute phase( 6~24h ), the latency phase( 7~42d, 14.5±5.9d ) and the chronic epileptic phase. By means of immunohistochemistry, we found: (1) clc-2 immune postive cells could be found in CA1-CA3 area of hippocampus, not found in the area dentate gyrus. 24h after seizure, the distribution and the optical...