Study Of The Effects And Mechanism Of The Uncaria Rhymchophylla. Jacks On Anti-vascular Remodeling And Inhibiting The Proliferation Of Vascular Smooth Muscle

Abjective:the effects and mechanism of the extract of Uncaria rhymchophylla. Jacks on anti- vascular remodeling and inhibiting the proliferation of vascular smooth muscle.Methods:(1)It was observed that the effect of extract of Uncaria rhymchophylla.Jacks on the SHR blood pressure,the changes of the 3 level of artery structure under microscope and physiology the level of the angiotesinâ…¡and calcium ion in the SHR aorta vascular smooth muscle in vivo,so that the mechanism of the total alkaloids of Uncaria rhymchophylla.Jacks of the reducing the SHR blood pressure and improving the vascular remodeling was studied. (2)The model of the proliferation of the cultivated vascular smooth muscle cells induced by angiotesinâ…¡was made,the examined index and mothods were used as following: the level of DNA synthesis inspected by MTT, the inhibition of the growth of the vascular smoosth muscle cells examined by the cell growth curve,the growth cycle of the vascular smooth muscle cells and the the level of calcium ions and c-myc gene expression in the cultivated cells observed by flow cyteometric analysis,Results: the blood pressure of SHR tails could be reduced by total alkaloid of Uncaria rhymchophylla.Jacks, compared with model group ,there was obvious difference (P<0.01). the tunica intima, tunica media and tunica advenitia of the aorta, renal and mesenric artery in the model group rats became thicker to different degrees;the ratio of the the thickness tunica media to diameter of the aorta, renal and mesenric artery was increased significantly,compared with normal control group,there was significant difference(P<0.01).compared with the control group,the ratio of the the thickness todiametre of tunica media of the aorta, renal and mesenric artery in total alkaloids of Uncaria rhymchophylla.Jacks group was decreased significantly(P<0.01).at the same time,the total alkaloids of Uncaria rhymchophylla.Jacks reduced the level of the angiotesinâ…¡and calcium ions in SHR arterys,there was difference and significant difference,compared with model control group(P<0.05,P<0.01).angiotesinâ…¡could promote the proliferation of VSMC obviously ; the medicines could inhibited the proliferation of the VSMC during12h and 24h, in medicine groups ;compared with model group,there were significant and very significant diffrence respectively(P<0.05,P<0.01). VSMC growth curves in each groups were recorded; after the intervening of the media contained medicines,the cell growth curves became lower, the inhibition effect in total alkaloids of Uncaria rhymchophylla.Jacks group was stronger than captopril group.It was found that G1 period became high(P<0.05) and S period became lower(P<0.01) in the total alkaloids of Uncaria rhymchophylla.Jacks group ,and total alkaloids of Uncaria rhymchophylla.Jacks blocked the transform from G0/G1 period to synthesis of DNA period,so that the VSMC proliferation was inhibited.Compared with the proliferation group induced by Angâ…¡,the level of Ca²⁺ ions expression in VSMC(static condition) was significantly lower and VSMC c-myc protein expression became lower(P<0.01, P<0.01).that possibaly means that total alkaloids of Uncaria rhymchophylla.Jacks inhibits the proliferation of VSMC through reducing the level of the calcium in the cells and the regulating the over–expression of the c-cym gene in the pathological condition.Conclusion:: total alkaloids of Uncaria rhymchophylla.Jacks has the function of reducing the blood pressure and improving the vascular remolding .the mechanism of reducing the blood pressure maybe concerns the reduction of the local level of Angâ…¡and Ca²⁺in the blood vessel.improving the vascular remolding perhaps conserns the decrease of the lowering the blood pressure and inhibiting the proliferation of the VSMC. The effect of inhibiting the proliferation possibaly conserns reducing the level of the calcium in the cells and the regulating the over–expression of the c-myc gene in the pathological condition.