In Vitro Experiment About Osteogenic Differentiation Of The Mesenchymal Stem Cell From Lipose And Bone Marrow Tissue In Rats

Charpter I The isolation and cultivation about bone marrow mesenchymal stem cells and adipose mesenchymal stem cells of ratsObjectives: Isolating the bone marrow mesenchymal stem cells and adipose mesenchymal stem cells, comparing the characteristics of two cells , providing the evidence for the adipose mesenchymal stem cells as the substitude of bone marrow mesenchymal stem cells.Methods: By the adherence , to isolate the bone marrow mesenchymal stem cells and adipose mesenchymal stem cells , to compare the characteristics of the two cells , including obtain the quantity of the cells , the morphos of the cells , the rate of adherence , cell kinetics , cell surface marker . To analysis the data .Results: (1)In per gram tissue, bone marrow mesenchymal stem cells is (2.23 ±0.35 )×10~5/g, adipose mesenchymal stem cells is (2.66 ±0.75)×10~5/g, by the two sample t test, t= —1.446, P=0.179>0.05, no sigmificant difference between two cells, wo can think the harvesting of the two cells is fairly. (2)The morphous of two cells are chord shape , like fibroblast. (3)By the analysis of linear correlation, coefficient correlation r is 0.999, coefficient of determination r~2 is 0.997, (F= 5862.949, P<0.001), we can think that there are linear correlation between the two adherences, the adherences are similar at different time, with the trend of increase by degress . (4)The reproductive activity are fairly between the adipose mesenchymal stem cells and bone marrow mesenchymal stem cells, the postdigestive 2nd and 5th generation cells have 6-9 days log phase growth after the latent period about 24-48 hours, then transit to the platform period. (5 ) By flow cytometer and immuocytochemistry, bone marrow mesenchymal stem cells and adipose...