Laboratory Diagnosis Of SARS And Application Of DNA Microarray On SARS Detection

Severe acute respiratory syndrome (SARS) caused by SARS coronavirus (SARS-CoV ) infected, found firstly in Guangdong in Nov. 2002, is a new acute infectious disease. The outbreak of SARS during the spring of 2003 spread quickly to 32 countries or areas all over the world.There were more than 8000 reported SARS cases, more than 700 death cases. In China, There were 5327 reported SARS cases(1512 cases in Guangdong ), 349 death cases(58 cases in Guangdong). During the spring of 2004, there were 4 newly SARS cases in Guangdong, besides, several SARS infecting accidents happened at lab in Taiwan, Sigapore,China. SARS has become a new infectious disease which seriously threatens human health.It is difficult for SARS diagnosis only by clinical symdrome because the similar symdrome among respiratory virus disease, so laboratory examinations are very important for SARS diagnosis.This research includs following contents: Isolation and identification of SARS pathogen; Comparison of results between laboratory test and clinical diagnosis for SARS cases; Study on prevalence of SARS-CoV antibody in different population in of Guangzhou Province and the relationship between wild animals and SARS-CoV; Laboratory diagnosis for 4 new SARS cases in Guangdong province during Dec, 2003 to Jan, 2004; Application of SARS-CoV DNA microarray on detection of SARS; Double restriction fluorescent labeling method for Microarray applications on trace DNA samples. The results are summarized in followings.