Experimental Research On Smooth Muscle Cell Proliferation And Intimal Hyperplasia In Autogenous Vein Graft

With severe major arterial injuries caused by either blunt or bullet in which a bridge or conduit is required, it is usually suggested that a vascular graft be needed. Autogenous vein graft has been applied to wide ranges of revasculature of limbs, heart and brain taking into consideration of its not only first-choice property but evaluational criteria for reference in other materials grafted. There are still however, fairly occlusions remained in grafted veins, which is largely due to excessive intimal hyperplasia besides other external factors with operation related. It is currently nuclear that the fate and trend of microstructural components in thickened intima at different functioning time post surgery. There is not a quantitative method as yet for specific and accuracy in analyzing smooth muscle cell (SMC).The medicament results to intimal hyperplasia are not satisfactory. This study was to quantitatively measure alterations of SMC, endothelial cell and leukocyte involved in the thickened intima in graft, which based on the animal veins-to-arteries model, immunohistochemistry and mRNA in situ hybridization techniques combined with genuine color scanner computer image analysis system, and to observe a method quantitatively examining SMC. In this study, we also designed a soft extravascular model(SEM) with β -radioactive ray to investigate its effects on intimal hyperplasia and SMC proliferation. The results are as follows:1. In situ molecular hybridization with ETA cDNA gene probe is an accurate and specific method in quantitatively analyzing SMC in IH. Endothelin may play an important role in development of IH in autogenous vein graft.