| Analgesia has always been the main subject under investigation to endodon-tists. This study was conducted to probe into the possible mechanism of toad venom in fast indolent pulpotomy and pulp devitalization as well as the origin of its most effective component. The possibility of using toad venom as analgesia in pulp treatment was also discussed. The results were as follows:1. Toad opisthotic gland secretion (TOGS), toad venom alcohol extract (TVAE) and chloroform extract (TVCE) were more effective than Lidocaine in either surface or infiltration anesthesia. TOGS paralyzed pulp more quickly and intensely than TVAE and TVCE paste did. After dressing with TOGS paste for 4 hours, CGRP—positive nerve fibers in pulp tissue were damaged and decreased in number. With a rate of success at 96. 9%, the pulpotomy analgesia of TOGS plus Dicaine (4:1) was significantly more effective than that of TOGS, TVAE or TVCE plus Cocaine (2:1) respectively.2. As to cytotoxicity, TOGS and TVAE were stronger than Arsenic, while TVCE was similar to Arsenic. At the early stage of dressing with TVAE, pulp blood vessels changed sharply with a result of total pulp necrosis 3～5 days later, a little later than that with Arsenic. The pattern of necrosis included hemor-rage and liquefaction. At the early stage of TOGS or TVCE dressing, no obvious pulp blood vessel changes were observed and total pulp necrosis occured 5 days later, prevailed by liquefaction -, After TOGS dressing for 1 hour, ultrastructure changes were observed in pulp nerve fibers and cells, capillaries contraction was... |
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