Extraction, Purification, Physico-chemical Characterization And Biological Effects Of Capsules From Oral Anaerobes On Human Gingival And Periodontal Ligament Fibroblasts And Osteoclasts In Vitro

Capsules are additional surface components possessed by many bacteria and of importance in pathogenesis of infectious diseases, such as periodontitis and pneumonia. The strong pathogenic effects of thses bacteria components have now brought to considerable attention, however the exact role has not yet been clarified and studies of their involvment in oral infectious diseases have been sparse. An attempt was therefore made to extract and purify capsules from three oral anaerobes, and to study their physico-chemical characterization and potential biological effects on fibroblasts and osteoclasts.Part I1. Extraction and purification of capsule from Pg. Capsule from Pg was extracted with saline and purified by HPLC. Preliminary chemical analysis of the purified capsule indicated that it may be glycoprotein, the ratio of protein and carbohydrate was 8.9:1, the yield from lyophilized bacteria was 0.22% and its endotoxin content was extremely low. Electron microscopic examination revealed that isolated material was from the bacteria surface components, and absence of gross cell disruption during extracting process.2. Comparison of characterization of three capsules on HPLC. Gel filtration on HPLC revealed that most of protein and carbohydrate of capsule from Pg eluted as two major peaks and three major peaks from Pi and...