Study On Myogenic Capability Of DMSO-Induced Embryonic Stem Cell Transfected With MyoD In Mouse

Fibrotic degeneration or scar proliferation of muscle will occur after long-term denervation or injury. As a result, the morphological and structural as well as the functional defects of the damaged muscle can be clearly observed in skeletal and cardiac muscles. Both kinds of muscle cells, as we know, have come to their terminal differentiation and have no capacity to regenerate. Recent studies have proved that the dormant muscle cells namely skeletal muscle satellite cells possess potentialities to be activated when the muscle is denervated or injured and participate in muscle repair. However, the number of this cell is far from enough to meet the need for muscle repairing. Tissue engineering technique has been successfully applied in repair of various tissues in vivo. It reminds us whether we can find a cell resource and implant the cells into the injured muscle to accomplish muscle repair and thus to avoid facing the trouble of satellite cell inadequacy.The embryonic stem (ES) cell is derived from the preimplantation blastocyst inner cell mass and proved to be omnipotent stem cell. It can differentiate automatically into various muscle cells, neuron cell, glia cell, and so on in vitro. However, ES cell alone is not suitable for skeletal muscle repair because it is pluripotent in differentiation. So we have to take into account of how to control its differentiation before...