A Study Of Intervention And Its Molecular Mechanism Of Salidroside On Dermal Fibroblasts In Photoaging

Salidroside is a main ingredient of chineseherb Rhodiola. It will be proved that salidroside can prevent dermis from photoaging, and its mechanism will be studied in this paper. The change of the apoptosis rate of fibroblasts and effect of salidroside and will its mechanism will be studied in this paper. Photoaging was thought to be due to the quantitative and qualitativealterations of structural proteins of the dermis induced by ultraviolet irradiation. Histological analysis showed the degeneration of collagen fibers and changes in the ratio of different types of collagen were caused by ultraviolet irradiation. It is indicated that dermal fibroblasts played an important rolein photoaging. A study on salidroside s impact on photoaging. This study will probe salidroside's effect on fibroblasts under UVB and UVA and explored the molecular mechanism of UV irradiation on fibroblast in photoaging in 2 parts.Object Part I: To probe salidroside's effect on fibroblasts under UVB and UVA on the basis of the apoptosis rate. Part II: To probe the molecular mechanism of the effect of salidroside from the point of view of molecular biology.Methods 1.To observe the changing of fibroblasts by UV was explored and impact of salidroside on it. The apoptosis rate of control group and experiment fibroblasts was detected by flow cytometer. To probe the effect of salidroside on fibroblasts growth after UV irradiation. 2. The mRNA expression levels of Fas and Bcl-2 were determined by RT-PCR.To probe the relationship between salidroside and apoptosis gene. 3. The mRNA expression levels of TNF-a and IL?1 were determined by RT-PCR. To probe the relationship between salidroside and cell factor. 4. The caspase-3 and caspase-8 protein were detected by colorimetric analysis.Results 1. The fibroblasts were great damaged after UV irradiation. It didn't change after adition of salidroside. The apoptosis rate was observed in both fibroblast groups treated with UVA or UVB respectively. After treated with salidroside, the apoptosis rate was decreased obviously. It s showed that salidroside can protect fibroblasts from apoptosis casused UV. 2. The mRNA level of Fas was both elevated after UVA or UVB irradiation and was inhibited by salidroside treatment. The mRNA expression of Bcl-2 was both decreased after UVA and UVB irradiation, and was elevated by salidroside treatment. The salidroside treatment could elevated the mRNA change of Bcl-2 induced by UVA irradiation. 3. The mRNA expression of TNF-αand IL-1βwere obviously increased after UVB and UVA irradiation comparing with thecontrol group. After treated with salidroside, the mRNA expression were both decreased. The salidroside treatment could relieve the skin damage induced by UVB and UVA through decreasing thesecretion of TNF-αand IL-1βmRNA expression. 4. The secretions of caspase-3 and caspase-8 were obviously increased after UVB and UVA irradiation comparing with thecontrol group. After treated with salidroside, the secretions were both decreased.Conclusions All the results indicated that UVB and UVB irradiation could induce fibroblast apoptosis, which could decrease the quantity of fibroblasts. The salidroside treatment could relieve the skin damage induced by UVB and UVA irradiation. The UVB and UVA could induce fibroblast apoptosis by decreasing-the mRNA expression of Bcl-2 and increasing the mRNA expression of Fas, TNF-αand IL-1β. The secretions of caspase-3 and caspase-8 were obviously increased after UVB and.UVA too. All of them could decrease the quantity of fibroblasts. The salidroside could restrain photoaging by inhibting these changes. This work may contribute to a better understanding themolecular mechanism of UVB and UVA irradiation on fibroblast in photoaging.