Correlation Between Inflammatory Factor And Blood Stasis Syndrome In Cerebral Infarction In Humans

BACKGROUND: Blood stasis syndrome usually occurs in diseases related to thrombussuch as cerebrovascular disease, cardiovascular disease, peripheryphlebothrombosis or arterial occlusions. Inflammatory reaction participate theacute cerebrovascular diseases course. These inflammatory cell factorparticipate the procedure of the acute cerebrovascular diseases. So we presumethat inflammatory reaction may be correlated to the emergence of blood stasissyndrome.OBJECTIVE: Analyzing the age, blood pressure, blood examination, blood fat,inflammatory reaction cell factors between the cerebral infarction group andthe control group. Survey the relationship between these targets and the cerebralinfarction. To analyze blood stasis syndrome in both the cerebral infarctiongroup and the control group that without cerebral infarction was conducted toexplore the correlation between inflammatory factor and blood stasis syndrome.METHODS: This subject is an observatory and controlled study based on patientswith cerebral infarction and without cerebral infarction. All these participantswere hospitalized at the Dongzhimen hospital which was the first affiliatedhospital of Beijing University of Traditional Chinese Medicine. We collectedthe name, age, sex, and other information from each participant, including bloodexamination, blood fat and index of blood coagulation and so on. We usequestionnaire to evaluate each participant's pattern identification. We alsodraw vein blood 3ml from each participant at the state of empty stomach for makingsera. All sera were hold in the-70 centi-degree refrigerator and were detectedtogether in pharmacological laboratory in Dongzhimen hospital. IL-1β,IL-6,TNF-α,TGF-β,sICAM-1 and sVCAM-1 were detected with the enzyme linkedimmunosorbent assay(ELISA) kit of IL-1,IL-6,TNF-α,TGF-β,sICAM-1 and sVCAM-1by the method of ELISA. At last, we finished our database and statisticalanalysis.RESULTS: Age, as an demography information, is very important in this subject.We divided all participants into two groups. 70 years old is selected to be thedemarcation. In the group that with the participants in age＜70 years old, thereare 35 patients with blood stasis syndrome and 32 patients without blood stasissyndrome. In the group that with the participants in age≥70 years old, thereare 44 patients with blood stasis syndrome and 19 patients without blood stasissyndrome. Through Pearson chi-square criterion, we get the result P＜0.05. Thecorrelation test shows that Pearson coefficient correlation between age andblood stasis syndrome score is 0.185, P＜0.05, accompany with the increase ofage, the blood stasis syndrome score is increased. PT is an blood vessel Riskfactor. The PT level in patients with blood stasis syndrome is 12.16±0.95(s).The PT level in patients without blood stasis syndrome is 12.52±1.03(s). t test result shows P＜0.05. Patients with blood stasis syndrome have high PT levelcompare with the patients without blood stasis syndrome. Analyzing theinflammatory factor, the Pearson coefficient correlation between IL-1βleveland the blood stasis syndrome score is 0.354, P＜0.01. Spearman coefficientcorrelation is 0.305, P＜0.05. The result shows the positive correlation betweenIL-1βand blood stasis syndrome. The Pearson coefficient correlation betweenTNF-αlevel and the blood stasis syndrome score is -0.253, P＜0.05. Spearmancoefficient correlation is -0.264, P＜0.01. As the same, The Pearson coefficientcorrelation between TGF-β1 level and the blood stasis syndrome score is -0.235,P＜0.05. The result shows inverse correlation between TNF-αlevel/TGF-β1 leveland the blood stasis syndrome score. The Spearman coefficient correlationbetween sICAM-1 level and the blood stasis syndrome score is 0.342, P＜0.05. Thereis a relationship of positive correlation between sICAM-1 and blood stasissyndrome in cerebral infarction too.CONCLUSION: The correlation factor of blood stasis syndrome is age. The possiblecorrelation factors of blood stasis syndrome are prothrombin time. Inflammatoryfactors IL-1β,IL-6,TNF-α,TGF-β,sICAM-1,sVCAM-1 are participate the cerebralinfarction course. The result shows the positive correlation between IL-1βandblood stasis syndrome. It shows inverse correlation between TNF-αlevel/TGF-β1 level and the blood stasis syndrome score too. Also there is a relationshipof positive correlation between sICAM-1 and blood stasis syndrome in cerebralinfarction. Inflammatory factor participate the cerebral infarction course. Andshow the different correlation with the blood stasis syndrome. Inflammatoryfactors as a biological factor maybe take part in the forming course of bloodstasis syndrome in patients with cerebral infarction. Blood stasis syndrome asan important pattern, may have more complicated mechanism in the course of happen,develop and form. (The project was supported by National 973 programe)...