Research On The Relevant Between Spleenashenic Syndrome And Mitochondria Gene Polymorphism, Gastrin, ATPase

The spleen is the core of doctrine of visceral state and the spleen controlling digestionis one of the most important theories of traditional Chinese medicine. The splenasthenicsyndrome is very frequent in clinic. In decades years, spleen-deficiency syndrome has beena hot subject people have been studying. My tutor put forward the theory that the spleen hasclose relation with cell mitochondria. Based on the theory and previous studies and drawingassistance from gene and molecular biology technology, from the theory, the clinic and theexperiment, we studied the etiopathogenisis of splenasthenic syndrome and the relationshipbetween splenasthenic syndrome and gene, gastrin, ATPase.1. Literature reviewFrom the analyses of ancient literature we consider that the etiopathogenisis ofsplenasthenic syndrome is innating deficiency, damp pathogen obstruction the spleen,melancholy, anxiety and overstrain, eating and drinking without temperance, damage to thespleen and stomach from bitter and cold drugs. Because of these etiopathogenisis, spleencann't control digestion and spleen failing to control blood. So nourishing qi to invigoratespleen is the therapeutical principle of splenasthenic syndrome.About the modern studies of splenasthenic syndrome, most scholars studied from theconditions of pathopoiesis, pathways, material foundations. They considered thatsplenasthenic syndrome can result in the weaknesses of digestion and absorptive functionand energy metabolism, the changes in blood rheology and minor elements, the loss of theimmune and the endocrine and the generation functions, the reduction of gut hormonesecretion etc.In decade years, most scholars uniformly considered that the decrease ofmitochondrial amount, the break of cristae, the changes of fine structure and the decrease ofrespiratory chain enzymatic activity existed in splenasthenic syndrome. These theoriescoincided with my tutor's theory. The study about dependability between splenasthenicsyndrome and genes is in initial phase and related literature is insufficient. Some literaturesis about stomach cancer P53 gene and splenasthenic syndrome. Above studies manifested that the spleen and stomach is the center of various kinds of pathological changes onsplenasthenic syndrome,2. Clinical studyIn order to study the relation between splenasthenic syndrome and mitochondrial genemutation and expression, according to strict diagnostic and internalization and removingcriteria we sepatated 50 chronic gastritis and peptic ulcer patients to 4 groups: deficiency ofspleen-QI group, insufficiency of spleen-YANG group, asthenic splenoyin group,splenogastric hygropyrexia group. In order to search gene mutations related withsplenasthenic syndrome, we drawed patients'blood in early morning, detectedmitochondrial D-loop gene orders and compared with orders in genebank, detected thegene expressions.Result display:(1) 436 gene mutations in 46 sites were founded in 1528bp nucleic acid fragment. Theaverage mutation density is 1.12 per capita per 100 basic group. The base transition isleading variety, the next is gene insertions, gene absences and gene transversions.(2) C→T mutation was founded in 16223 site and its mutation frequency is 40%. Themechanism is not clear which maybe can result in splenasthenic syndrome.(3) The average aberration rate in deficiency of spleen-QI group is the tallest. It is higherthan the last 4 groups (P＜0.01 or P＜0.05).(4) The average aberration rate in peptic ulcer patients group is significantly higher thanchronic gastritis patients group (P＜0.01)(5) The gene expression of splenogastric hygropyrexia group is higher than splenasthenicsyndrome group (P＜0.05).(6) The gene expression of chronic gastritis patients higher than peptic ulcer patients, but ithas no statistics difference (P＞0.05).3. Empirical studyThe rats in the model group were fed with half full and perfuse with XiaochengqiTang(60g/kg) every day to build animal model of Deficiency of Spleen-energy. We detectedthe content of serum gastrin with radio-immunity method, the activity of Na⁺-K⁺-ATPaseand Ca²⁺-Mg²⁺ATPase of mitochontria in red cell envelope, liver and skeletal muscle.Result display:(1) The GAS content in model group of spleen deficiency is lower than 4 groups treatedwith the traditional Chinese medicine and normal control group. It has significantdifference (P＜0.01). The GAS content in groups treated with the traditional Chinese medicine excluding Dangguibuxuetang group is higher than normal control group. It hassignificant difference (P＜0.05)(2) The ATPase activity in red cell envelope of model of spleen deficiency group is lowest.The ATPase activity in red cell envelope of the groups treated with the traditional Chinesemedicine excluding Dangguibuxuetang group is lower than normal control group (P＞0.05)and significantly higher than model of spleen deficiency group(P＜0.01). The ATPaseactivity in red cell envelope of Dangguibuxuetang group is elevated and higher than modelof spleen deficiency (P＜0.05)and lower than normal control group(P＜0.05).(3) The ATPase activity in liver cell mitochondria of model of spleen deficiency group islower than normal control group (P＜0.01). The ATPase activity of the 4 groups treated withthe traditional Chinese medicine are lower than normal control group and significantlyhigher than model of spleen deficiency group (P＜0.01 or P＜0.05). The elevated order:Dangguibuxue-sijunzitang group＞Huangqisijunzitang group＞Sijunzitang group＞Dangguibuxuetang group. The ATPase activity of Dangguibuxuetang group. The ATPaseactivity of Dangguibuxuetang group is significantly lower than normal controlgroup(P＜0.05).(4) The ATPase activity in mitochondria of skeletal muscle of model of spleen deficiencygroup is lower than normal control group and 4 groups treated with the traditional Chinesemedicine (P＜0.01 or P＜0.05). The ATPase activity of Huangqisijunzitang group issignificantly higher than normal control group(P＜0.05). The last 3 group treated with thetraditional Chinese medicine have no significant difference compared with normal controlgroup (P＞0.05).4. ConclusionGuided with prof. Liuyouzhang's theory "the spleen has close relation with cellmitochondria" and combinated clinical research with experimental research, we obtainobjective findings: gene polymorphism, genic abnormal expression, the decrease of gastrinand the activity of ATPase existed in genesplenasthenic syndrome. We elucidated theessence of splenasthenic syndrome from gene and molecular biology aspects. So wedevelop the local of the studies in past which were only concerned in systems, organs,organizations and provide objective experimental evidence for clinical treatment ofsplenasthenic syndrome.