Study Of Protective Effect And Mechanism Of C-type Natriuretic Peptide And Its Functional Receptor NPR-B On Diabetic Nephropathy

This study investigated STZ-induced diabetes rats, mesangial cells and endothelial cells as object to approach the protective effection of CNP and NPR-B recptor in diabetic nephropathy and their mechanism by the method of immunohistochemistry, immunofluorescent technique, ELISA, western blot, RealTime-PCR and so on. Presence of local autogenous regulation of CNP and NPR-B were found in diabetes rats renal. CNP and NPR-B showed different characteristic on renal cortex at different pathological period, with TongLuo recipe regulated their expression. TongLuo recipe contributed to the synthesis of CNP in cultured human umbilical vein cells. CNP inhibited the active NF-κB and restrainted the proliferation and phenotype converting induced by AGEs or ROS through NPR-B/cGMP pathway in cultured mesangial cells. These study suggesting that CNP and its functional receptor NPR-B may play a protective role on diabetic nephropathy, TongLuo Recipe can regulates their express.