Screen For Prognostic Factors Of Spinal Metastases And Establishment Of Cox Model

Objective To investigate the prognostic relevane of expression of tumor-related gene and clinical features in patients with spinal metastases and establish Cox model. Methods Expression of tumor-related gene such as Bcl-2,C-erbB-2,CyclinD1,p16,p27kip1,PTEN,p53,MMP-2,MMP-7,MMP-13,MMP-14,CD44v6,VEGF,nm23-H1 and OPN were detected by immunohistochemistry on tissue chips built with 128 specimens from 64 cases of spinal metastases from lung cacer, liver cancer and unknown primary. Cox model were estabished according to mutivariate analyses of survival data. Results Abnormal expression of all genes involved in this study were detected. No significance between expression and prognosis were found except for PTEN, p27kip1, MMP-14, CD44v6 and OPN. Visceral metastasis, number of vertebral involvement, pathological class, expression of CD44v6, OPN and PTEN entered the final Cox model for prognosis of spinal metastases. Conclusion Multiple genes involve in carcinogenesis and progression of spinal metastases. Visceral metastasis, number of vertebral involvement, pathological class, and expression of CD44v6, OPN and PTEN are major factors on prognosis of spinal metastases, and a high PI correlates with high risk and poor prognosis.