| Objective: To explore effect and mechanism of recombinant adenovirus viral interleukin 10 (Ad/vIL-10) in protection against brain ischemia. Methods: Constructed by Cre-LoxP system, Ad/vIL-10 was transferred into isolated basilar arteries from canine and isolated thoracic arteries from IL-10 knock-out (IL-10-/-) and wild type (WT) mice to examine its protective roles in vascular impairment by lysophosphatidylcholine (lysoPC). Meanwhile, protection of Ad/vIL-10 was examined against cultured cerebromicrovascular endothelial cells impaired by lysoPC and H/R also. Further, relationship between effects of Ad/vIL-10 on vasocontraction to lysoPC and expression of ENOS and HO-1 was explored to clarify mechanism of protective role of Ad/vIL-10. Results: Ad/vIL-10 significantly attenuated vasocontraction to lysoPC in isolated basilar arteries from canine and thoracic arteries from WT and IL-10-/- mice, and significantly decreased adhesion rate and adhesion molecules expression. Ad/vIL-10 significantly improved vasocontraction to lysoPC by up-regulating expression of eNOS and HO-1. Conclusions: Ad/vIL-10 protected against brain ischemia injury, which may be involved with induction of eNOS and HO-1. |
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