Study On Experiment Of Influence On Airway Remodeling Of Chronic Obstructive Pulmonary Disease With Chinese Medicine

Objective: study on experiment of influence on airway remodeling of chronic obstructivepulmonary disease with chinese medicineMethod:110 spragu-dawley rats were randomly divided into five groups:controlgroup,COPD group,COPD+Qideficiency syndrome+ puerarin/Tet group,COPD+Yindeficiency syndrome+ puerarin/Tet group,COPD+blood stasis syndrome+puerarin/Tetgroup,and Thalidomide group.The levels of TNF-αand IL-8 in BALF were measuredwith ELISA.The level of hydroxyproline in bronchial and lung homogenates wasdetermined by biochemistry method. And the level of hydroxyproline in serum was alsomeasured by biochemistry method. The activity of SOD in bronchial and lung homogenateswas determined.Lung tissue section stained by HE was observed to study themorphological alternations,inflammatory cells of the bronchial were counted.Thickness ofthe bronchial smooth muscle layer was measured by image analyzer.Expression andlocalization of TGF-β1 were observed in bronchi and lung tissues byimmunohistochemistry. TGF-β1 is tested expression in human lung fibroblasts with ELISA.Level of NF-κB be color on cellular chemisty.Result1. puerarin group(1) The level of the COPD model group's TNF-α,ET-1 in serum.IL-8 of BLAF is higherthan normal group, P＜0.01.density of SOD in COPD model group is higher than normal,P＜0.01. The numbers of inflammatory cells in the COPD model group was significantlyincreased compared to the control group(P＜0.01).The COPD model group's thickness of the bronchial smooth muscle layer was significantly increased compared to the controlgroup's thickness of the bronchial smooth muscle layer, P＜0.01. The relative content ofTGF-β1 in the epithelial cells of the bronchi in the COPD model group was significantlyhigher than that of control group,P＜0.01.(2)The level of the puerarin groups' TNF-α, ET-1 and IL-8 were lower than the COPDmodel group,P＜0.05, but ET-1 of COPD+blood stasis syndrome+puerarin, P＞0.05.Theactivity of SOD in the puerarin groups were significantly increased compared to the modelgroup, P＜0.05. The numbers of inflammatory cells in the puerarin groups weresignificantly decreased compared to the model group, P＜0.01. Thickness of the bronchialsmooth muscle layer in the puerarin groups were significantly decreased compared to themodel group, P＜0.01. the pulmonary arterial wall, P＜0.05, too.(3) There was no discrepancy in the all examinations among the puerarin groups.2. tetrandrine group(1)The level of the COPD model group's TNF-α, hydroxyproline in serum andhydroxyproline in bronchial and lung homogenates were higher than the control group'sTNF-α, hydroxyproline in serum and hydroxyproline in bronchial and lunghomogenates,P＜0.01.The numbers of inflammatory cells and fibroblast cell in the COPDmodel group was significantly increased compared to the control group(P＜0.01).TheCOPD model group's thickness of the bronchial smooth muscle layer(54.4+7.8um) wassignificantly increased compared to the control group's(27.2+1.8um),P＜0.01. The relativecontent of TGF-β1 in the epithelial cells of the bronchi in the COPD model group wassignificantly higher than that of control group(P＜0.01).(2)The level of the tetrandrine groups' TNF-αwas lower than the COPD modelgroup(P＜0.05).The levels of hydroxyproline in serum and lung homogenates in thetetrandrine groups were lower than the COPD model group(P＜0.01).The numbers ofinflammatory cells and fibroblast cell in the tetrandrine groups were significantlydecreased compared to the model group(P＜0.01).The thickness of the bronchial smoothmuscle layer was significantly decreased compared to the model group(P＜0.01). The relative content of TGF-β1 in the epithelial cells of the bronchi in the tetrandrine groupswere lower than that of model group(P＜0.01).(3)There was no discrepancy in the all examinations among the tetrandrine groups.3. Thalidomide group: anti-inflammtion and relived airway remodling be as well astetrandrine.but tetrandrine is superior to Thalidomide.4.expression of TGF-β1 in human lung fibroblast be decreased with tetrandrine,is low withconsistency of tetrandrine, P＜0.05.it can reduce NF-κB color on cellular chemisty.butpuerarin group cannot do as well as tetrandrineConclusion:1. puerarin has good effect on COPD model rats. It can lighten inflammatory action andoxidative stress and stave the process of the remodeling of the small airways.but cannotreduce expression of TGF-β1 and Level of NF-κB be color on cellular chemisty in humanlung fibroblast.2. Tet can decrease inflammatory action and the process of the remodeling of the smallairways.inhibition expression of TGF-β1 and NF-κB in human lung fibroblast3. Thalidomide anti-inflammtion and relived airway remodling be as well as tetrandrine.buttetrandrine is superior to Thalidomide...