Neurons The Differentiation Related Protein Dok5 Of And Map1b Function Research

Differentiation of neuron is a highly complicated process, including the gene expression regulation in multiple steps. Embryonal carcinoma and neuroblastoma are both formed during embryonal development, retinoic acid can induce them to differentiate into neurons. This model has been widely used to study the mechanism of neuronal differentiation.To find differentiation related proteins, 2D gel strategy was used to discover proteome alteration of SH-SY5Y induced by all-trans retinoic acid (ATRA). 73 differentially expressed protein spots were detected, in which the expression of 51 spots were down-regulated after differentiation and 22 spots up-regulated. Among which 40 proteins were finally identified by mass spectrometry. From the identified results, it was shown that multiple biological events were involved in ATRA induced neuronal differentiation, relate to energy metabolism, membrane trafficking, mRNA synthesis, protein synthesis, signal transduction etc. After differentiation the expression of c-Ret, a receptor tyrosine kinase was up-regulated. It was reported that Dok5 was the substrate of Ret, and can function in c-Ret-mediated neurite outgrowth. Dok5 was isolated from human fetal brain cDNA library in our lab and it has been found that the expression of Dok5 was up-regulated in P19 cells when it was induced by ATRA to differentiate into neuronal cells. But until now it is uncertain how dose it exert the function during neuronal differentiation.Dok5 is one member of Dok family. The carboxy-terminal parts of this family likely function as a molecular platform for signal complex assembly induced by activated PTKs. Finding out the interaction partners of Dok5 will be useful to understand its function in differentiation. We carried out a yeast two-hybrid screening, using the C-terminus of Dok5 as the bait and identified totally of 132 positive clones, representative of 27 proteins as direct Dok5 binding partners, including the light chain (LC1) of microtubule associated protein 1B (MAP1B). MAP1B is the first MAP to be expressed during the development of...