Investigate The Effect Of Yangxintang Medicated Serum On Differentially Expressed Genes And ST3GAL2 MRNA In An Oxidative Injury Model By Hydrogen Peroxide On Human Umbilical Vein Endothelia Cells

Objective:To observe the effects the formula of the serum with Yangxin Decoction on the oxidative damage of human umbilical vein endothelial cell'different genes expressions.To investigate its mechanics, then to provide academic warranties for preventing and curing the atherosc- lerosis early.Methods:Establishing the Cell models of the oxidative damage of human umbilical vein endothelial cell with the method of interferencing Hydrogen peroxide to human umbilical vein endothelial cell. Deviding the human umbilical vein endothelial cell into six groups random.①The blank control group ;②The model group;③the serum with Yangxin Decoction 2% group;④the serum with Yangxin Decoction 4% group;⑤the serum with Yangxin Decoction 6% group;⑥the serum with Yangxin Decoction 8% group.Observe cellular form, density Under inverted microscope. examine cellular viability with MTT.To observe the effects the formula of the serum with Yangxin Decoction on the oxidative damage of human umbilical vein endothelial cell'different genes expressions. To examine the expression of ST3GAL2mRNA with RT-PCR method.Result:1.The morphologic change of human umbilical vein endothel- ial cell: Normal umbilical vein endothelial cell's closely arranged, cobble- stone-like under the inverted microscope. The cell tunes to be round, falls off and the intercellular space broadens, the density reduces obviously, the cell looks like spindle-shaped, nucleus diffraction enhances after 100μmol L-1 hydrogen peroxide affects 2h;2.Model group was significantly lower than the blank control group in OD, statistically significant difference (P<0.01).Compared with model group, the serum with Yangxin Decoction 2% group has no significant difference(P>0.05) , the serum with Yangxin Decoction 4%,6%,8% group have significant differences(P<0.01). Compared with the serum with Yangxin Decoction 6%, the serum with Yangxin Decoction 2%,4%group have significant differences(P<0.01),the serum with Yangxin Decoction 8% group has no significant difference(P> 0.05);3.Model group compared with the blank control group upregulation, the serum with Yangxin Decoction compared with the model group downreg- ulation12 genes,Model group compared with the blank control group down- regulation, the serum with Yangxin Decoction compared with the model gro- up upregulation23 genes;4. The serum with Yangxin Decoction'expression of ST3GAL2mRNA 0.375 times for the model group,this is consistent with microarray results.Conclusions:1.The serum with Yang xin Decoction can reduce the inhibiti- on in which hydrogen peroxide effect human umbilical vein endothelial cell proliferation;2.The serum with Yangxin Decoction'different gene functions mainly in DNA binding,protein synthesis and transport,kinase,Platelet acti- vation,cell adhesion,etc;3.The serum with Yangxin Decoction can significa- ntly lower the oxidative injury model'ST3GAL2mRNA expression,which may be its function mechanism of its preventing and curing atherosclerosis.