Study On Antiviral And Antibacterial Active Components In Polygonum Taipaishanense And Polygonum Cillinerve

Searching antiviral and antibacterial active components from Chinese herbal medicine will be very helpful not only in providing theoretical evidence for developing new drug, but also in proper exploitation and utilization to resources of Chinese herbal medicine. This study mainly involved in 7 Chinese herbal medicines which belongs to Taibai Moutain area, Shaanxi Province at the basis of in vitro bacteriostasis tests. Selections on antiviral and antibacterial active fractions of Polygonum taipaishanense Kung and Polygonum cillinerve (Nakai) Ohwl in vitro and in vivo, separation and assessment of effective ingredients and other aspects were studied. The results were as following.1. All the Polygonum cillinerve ethyl fluoroacetate fraction (PCE), Polygonum taipaishanense ethyl fluoroacetate fraction (PTKE), Geranium wilfordii n-butanol fraction (GWN) and its ethyl fluoroacetate fraction (GWE) owned better virucidal activity with extensive antibacterial spectrum.2. The inhibition ratio of PTKE on proliferation of NDV in CEF are 32.49%. PTKEB had effect of directly elimination, anti- absorption and inhibiting proliferation on NDV in CEF (inhibition rate were 90.52%, 65.23% and 61.21%, respectively), there are obvious dosage-effect relationship between PTKEB and anti-NDV inhibitory rate. PTKEB can inhibit the proliferation of NDV in chicken embryo with extremely significant difference between herbal toxin mixed group and virus control(P<0.01). PTKE can inhibit E. coli, S. aureus, Salmonella, Pasteurella and S. agalactiae in vitro (their MBC were 100, 25, 50, 25 and 50 mg/mL respectively), and its isolates-PTKEA, PTKEB and PTKEC had inhibitory effect on S. aureus, Salmonella and S. agalactiae, but no antibacterial effect on E. coli and Pasteurella.3. PTKE could inhibit not only the diarrhea of mouse caused by Castor Oil and Senna Leaf extreme markedly (P<0.01), but also the heightening of capillary permeability located in rabbit intestine mucosa caused by Acetic Acid (P<0.01). In addition, it also could extremely inhibit heightening of capillary permeability located in abdominal cavity and in skin caused by acetic acid injected intraperitoneally and xylene injected intradermally respectively(P<0.05). The experimental groups were shown significant difference or the most significant difference compared with the contro(lP<0.05 or P<0.01)when added PTKE at different concentration to drinking water. The developmental state of histological structure of immune organs in experimental group were also better than it in control group. There were most significant difference on lymphocyte proliferation of spleen, thymus, bursa of Fabricius and peripheral blood between the PTKEB group at concenteation of 7.81μg/mL and the contro(lP<0.01). PTKE could promote the growth of chicken, but no evident trends could be seen both in reducing the rate of forage to meat and in increasing the transformation efficiency of forage.4. No obvious inhibitory effect could be observed in chloroform extract fraction of Polygonum cillinerve (PCC), emodin (EM) and physcione (PH) on proliferation of NDV in CEF. The inhibitory rate of PCE and its isolates PCEA on proliferation of NDV in CEF were 39.12% and 72.33%, respectively. PCEA can also inhibit the proliferation of NDV in chicken embryo with significant difference in hemagglutination titer between prevention group or herbal toxicity mixed group and virus control group(P<0.05), but no difference could be seen between treated group and virus control. All the minimal bactericidal concentration (MBC) of PCE to 9 bacteria were lower than 100 mg/mL. PCC had some virucidal effects on these bacteria. The MBC of Emodin on Salmonella (chicken), S.suis, S. agalactiae (cow), S.epidermidis (cow) and S. aureus (cow) were 5, 2.5, 2.5 , 2.5 and 1.25 mg/mL, respectively. The MBC of PCEA to Salmonella (chicken), S.suis, S. agalactiae (cow), S. epidermidis (cow), and S. aureus (cow) were 24, 24, 12, 6 and 6 mg/mL, respectively.5. EM, PH, PCEA, PCEB and PTKEB could eliminate TGEV directly, and their inhibitory rate were all more than 71.28 %. PCEA. PCEB and PTKEB could prevent the intracellular duplication of TGEV in ST cell, their inhibitory rate on biosynthesis of TGEV were 82.65 %, 85.74 % and 54.04 %, respectively. EM, PCEA, PCEB and PTKEB had adsorbing protection to infected cell by TGEV (their inhibitory rate were 44.85%, 69.07%, 83.08% and 48.28%, respectively). The inhibitory effect of PCEA, PCEB and PTKEB at different concentration on biosynthesis of TGEV in ST cell enhanced as increase of dosage.6. According to analysis by system pretesting, chromatographic separation and LC-MS,we detected Catechin and 3′,4′,5,7-Tetramethoxyflavone from PTKEB, while Anthraquinone including Emodin, Physcion, Emodin-8-β-D-glucopyranoside and Stilbenes containing Resveratrol and Piceid from PCC and PCE. These compounds were main active components related to antivirus and antibacteria.