Study The Effect Of Huoxue Dingchuan Soup To Asthmatic Airway Remodeling In Mice

Objective:Observed the relative content of plasma MMP-9 and TIMP-1, the change of plasma TGF-β1 and lung morphology in Balb/c mice, so as to explore the effect and mechanism of Huoxue Dingchuan Soup(HDS) to MMP-9/TIMP-1 balance and asthmatic airway remodeling.Methods:Balb/c mice 70 were randomly divided into normal group, model group, western medicine group, HDS low-dose group and high-dose group. Asthmatic airway remodeling model was established by repeated inhalation of ovalbumin. The normal group and model group administrated water, the other groups respectively administrated the corresponding medicines. At the end, determined plasma MMP-9,TIMP-1 and TGFβ1 by ELISA, observed the pathomorphology of lung under light microscope.Results::The pathological changes in lung tissue showed that HDS can significantly reduce the infiltration of inflammatory cells, alveolar wall edema and thickening of bronchial smooth muscle, can inhibit the occurrence of airway remodeling; model group compared with the control group, the level of MMP-9 and TIMP-1 except the normal group were significantly increased, after treatment they were significantly decreased, in which western medicine group and HDS high-dose group have significant difference comparing with the model group (P<0.01); In addition to the normal group at the same time, the other groups of MMP-9/TIMP-1 are imbalance in the ratio, the model group of TIMP-1 over-proportional increase eventually lead to an imbalance; plasma TGF-β1 results showed that compared with the normal group, TGF-β1 levels of model group were significantly increased, there is significant difference between the two groups (P<0.01), after treatment, the above indices of high-dose group and western medicine group decreased significantly and were significant differences compared with the model (P <0.01), while the high-dose group and western medicine group were no significant difference.Conclusion:HDS might suppress asthmatic airway remodeling by regulating the level of MMP-9, TIMP-1, TGF-β1 in the lung tissue of asthmatic airway remodeling mice and MMP-9/TIMP-1 balance.