The Influence Of Dahuang Huanglian Xiexin Decoction And Lizhong Pill On The Gastric Ulcer Model Induced By Indomethacin In The Cold Or Heat Syndrome Rats And The Rules Of Recipe Corresponding To Syndrome

ObjectTo establishing the model of gastric ulcer induced by indomethacin combined with cold or heat syndrome, and to exploring its forming mechanism,the partial mechanism of action of dahuang huanglian xiexin decoction and lizhong pill and the rules of recipe corresponding to syndrome. MethodAdopting cold factor (ice water +0.3mol/LNaOH),heat factor(capsicum+ 60﹪ethanol) combined with indomethacin intraperitoneal injection, to establish the model of gastric ulcer combined with cold or heat syndrome , which is the model of"disease combined with syndrome".Then selecting two contradictory nature prescriptions(Dahuang huanglian xiexin Decoction,Lizhong Pill)to cross-fire treatment. Detecting the indexes of every groups, SOD,MDA,VEGF,bFGF,IL-8,TNF-α,PGE₂,TXB₂,NT,GAS.Results1,Each making-model factor can accelerate the destruction of gaster-mucosa, promoting the formation of ulcer at different degrees through increasing the expression of IL-8 and TNF-α, increasing the contents of Gas and MDA, accelerating the release of TXB₂, decreasing the energy of SOD and the synthesis- release of PGE₂, inhibiting the secretion of NT. Simultaneously, each factor also can increase the contents of VEGF,bFGF,to promote the recovery of gaster-mucosa damage.2,Heat nature prescription Lizhong Pill produce a marked effect to cold mode, and cold nature prescription Dahuang huanglian xiexin Decoction produce a conspicuous effect to heat model, which indicate the selectional syndrome-treatment potency. The two prescriptions can recover the gaster-mucosa damage of corresponding model through increasing the energy of SOD, decreasing the contents of MDA, increasing the release of VEGF,bFGF, inhibiting the generation of inflammatory factors IL-8,TGF-α, decreasing the synthesis of Gas,TXB₂, inhibiting the secretion of Gas, increasing the release of NT,PGE₂。 3,The degrees of increasing the expression of IL-8 and TNF-α, and of increasing the contents of MDA and Gas indicate more strikingly in heat models. The degree of increasing the release of TXB₂ manifests more obviously in cold models. ConclusionOn the basis of the theory of"prescription-syndrome corresponding", through the method of"infering syndrome by prescription"verifying the success of duplicating"disease-syndrome combination model", that is the model of gastric ulcer induced by indomethacin combined with cold or heat syndrome. Elucidating the partial forming mechanism of this model, and the microcosmic variability between cold model and heat model.