The Cross-section Study About Predictors Of Cardiovascular Events In Beijing Population

Background:The levels of high-sensitivity cardiac troponin T (hsTnT) was useful predictor for cardiovascular events. It provided useful information in population with chest pain, even at levels well below the detection limits of previous assays. However, the association between the levels of hsTnT and the predicted cardiovascular risk remains unclear. The arterial stiffness was another useful predictor for cardiovascular events. The peroxisome proliferator-activated receptor (PPAR) and peroxisome proliferator-activated receptor-y coactivator-la (PPARγC1a) play an important role in the metabolic process of glucose and lipid. Whether single nucleotide polymorphisms (SNPs) of these gene accout for arterial stiffness, it was still unknown.Methods:The hsTnT levels were measured from 1,365 community-based middle-aged to older adults. The association between cardiovascular risk factors and hsTnT level was examined. The association between hsTnT levels and predicted coronary heart disease (CHD) risk and predicted general cardiovascular disease (CVD) risk were analyzed. After purification of genomic DNA samples from clotted blood by a new method, three valid SNPs, including rs1053049, rs1800234 and rs8192678 in the PPAR and PPARyCla gene were genotyped in 1,374 subjects (530 patients and 844 controls) by TaqMan allelic discrimination assays. The association between cardiovascular risk factors and SNPs with the arterial stiffness were analyzed.Results:The detectable and elevated hsTnT levels were obtained in 719 subjects (52.7%) and 150 subjects (11.0%), respectively. In multivariable analyses, independent associations were found between hsTnT level and male sex, fasting glucose, N-terminal pro B-type natriuretic peptide, and estimated glomerular filtration rate. After adjustment for confounding factors, strong and graded increases in high predicted CHD risk (10-year risk≥20%) (adjusted OR per unit increase in the natural logarithm of the hsTnT levels,2.134; 95% CI,1.309 to 3.480; P=0.002) and high predicted general CVD risk (10-year risk>20%) (adjusted OR,2.362; 95% CI,1.583 to 3.524; P<0.001) were found. The higher predicted cardiovascular risk were found in intermediate and high hsTnT groups too. DNA samples purified from clotted blood were successfully performed through polymerase chain reaction, real time polymerase chain reaction, and melt curve analysis. Age, hypertension and diabetes mellitus were the main factors for increased arterial stiffness. There were no association between SNPs of rs1053049, rs1800234 and rs8192678 and the increased arterial stiffness among this community-based population. Haplotypes were analysed with multiple loci and there were no association between haplotypes and the increased arterial stiffness too.Conclusions:The hsTnT levels were associated with several cardiovascular risk factors in this community-based population, and it coincided with predicted cardiovascular risk. Determining hsTnT levels may aid clinicians in identifying patients with increased cardiovascular risk in the general population. The present study did not find association between SNPs of PPAR and PPARγC1a with the increased arterial stiffness in this community-based population.