The Expression And Function Of Glycogen Phosphorylase In Liver Cancer

Liver cancer is the fifth most commom cancer worldwide and the third most common cause of death from cancer, resulting in more than 600,000 deaths each year. Its main forms are hepatocellular carcinoma (HCC) and Intrahepatic cholangiocarcinoma (ICC). The development and progression of HCC or ICC is a complicated process involving multiple genes and several transforming steps. The mechanisms underlying the development of HCC/ICC remain unclear. Therefore, searching for new HCC/ICC associated molecules may give some clues to study the mechanism of HCC/ICC.Glycogen phosphorylase (GP) plays a central role in the mobilization of carbohydrate reserves in a wide variety of organs and tissues, and has a long history of bing investigated, starting in the 1930s. Mammalian GPs are found in three major isoforms, i.e., muscle, liver, and brain, which can be distinguished by functional and structural properties as well as by the tissues in which they are predominantly expressed. The physiological role of GP is poorly understood in liver cancer.This study revealed that PYGB/PYGL played an important role in the development and progression of HCC/ICC, indicating its roles as a potential therapeutic target in HCC/ICC. Hepatocellular carcinoma (HCC) is the fifth most commom cancer worldwide and the third most common cause of death from cancer, resulting in more than 600,000 deaths each year. The development and progression of HCC is a complicated process involving multiple genes and several transforming steps. Up to now, though over 20 genes have been found to be relative to hepatocarcinogenesis, such as P53,P28GANK,Rb,ras,c-myc,c-fos,c-jun et al, the mechanisms underlying the development of HCC remain unclear. Therefore, searching for new HCC associated molecules may give some clues to study the mechanism of HCC.p300 was originally identified using protein-protein interaction assays with the adenoviral E1A oncoprotein. p300 has been implicated in a number of diverse biological functions including proliferation, cell cycle regulation, apoptosis, differentiation and DNA damage response. p300 and CBP have been implicated in embryonic development and cancer. p300 is highly conserved and functionally related transcription coactivators and histone/protein acetyltransferases. Recently, growing attention has been paid to its role in the initialtion and progression of various cancers. However, the role of p300 in HCC has been rarely investigated. In this study, we found that the expression of p300 was significantly up-regulated in both cell lines and clinical tissue sample of HCC, and associated with poor prognosis. In vitro, we can see clearly that p300 promote proliferation of HCC cell.This study revealed that p300 played an important role in the development and progression of HCC by promoting the survival of cancer cells, indicating its roles as a potential therapeutic target in HCC.