| The application of Asiatic wormwood has a centuries-old history in China. Chinese medicine thinks that Asiatic wormwood can recuperate QI and blood, warm meridian and channel, drive out cold and dampness, and stop crymodynia. Asiatic wormwood has a long history in preventing pestilence. The pharmacology research of Modern medicine indicates that Asiatic wormwood is a broad spectrum antibiosis medicine. Asiatic wormwood can restrain various virus and germ, and can prevent and cure some respiratory system diseases.The moxibustion or moxa treatment is an important part of national medicine. It is a rich experience of ancient Chinese Laboring people drawing from long period's battles against the disease. And it is a kind of treatment, which can prevent and cure disease and make people stronger and healthier. The moxibustion burns the acupoint or the pain-point through moxa or other medicines to warm meridian and channel, recuperate QI and blood, Coordinate yin and yang, and strengthen body resistance and eliminating evil. The moxibustion has the function of curing diseases, preventing diseases, keeping in good health and improving looks. People pay attention to the moxibustion for a long time.Because of it's high safety and functions of keeping in good health and improving looks, the moxibustion is spreaded widely. Now the curative effect is a prerequisite to the application and dissemination of treatment. The effect appears through natural capacity of medicine and proper amount of application.This subject starting from the moxibustion material through experiment method to analyze moxa pharmacological characteristics. By this way, we want to provide some data support to the mechanism of moxibustion study and improve the effectiveness of moxibustion therapy.Objective:Through the Artemisia Argyi of different years from Qichun volatile constituents, to detect the determination of moxibustion therapy pain threshold and fatigue in mice. And observe the influence of the human body surface temperature under mild moxibustion change from different perspectives, and explore different moxibustion material effect significance from the aspect of temperature.Method:Experiment 1:through the research different years of moxa wool (1-year and 5-year)volatile oil content by gas chromatography-mass spectrometry, comparing different years of moxa wool components.Experiment 2:Laboratory mice divided into six groups as follows:control group, 1-year of Ai group, Naphtha group A,5-year of Ai group, Naphtha group B, positive group, and observe different hot plate processing methods of analgesic experiments in mice pain threshold value influence.Experiment 3:Laboratory mice divided into five groups as follows:control group, 1-year of Ai, Naphtha group A, 5-year of Ai, Naphtha group B, and observe influence from different processing methods of mice swimming in the experiment.Clinical Research:The 90 volunteers randomly devided into three groups as follow:one-year 30 cases of Ai, three-year 30 cases of Ai, five-year 30 cases of Ai.Acupoint surface temperatures of three different years of warming moxibustion (1-year,3-year,5-year) were measured in 90 volunteers, and then a comparison of surface temperature changes in right acupoint(Feishu) was made among pre-moxibustion, moxibustion-processing(2,4,6,8,10min). The sense of comfort and the acceptance of the moxibustion were surveyed after the experiment.Results:Different years of moxa wool the chemical composition of essential oil analysis:The volatile oil composition analysis comparison were identified by gas chromatography-mass spectrometry (GC-MS):1-year of Ai found 52 species chemical composition, 5-year of Ai found 50 kinds of chemical composition; 1-year of Ai elliott volatile oil content is in more than 5-year of Ai.The mice of analgesia experiments analysis:Comparing with all groups before treatment, there was no significant difference(p>0.05). Comparing with the control group after treatment, positive group, moxibustion group and naphtha group all have significant differences (P<0.05); Comparing with the positive group, moxibustion group and naphtha group have significant differences (P<0.05); Comparing with the moxibustion group A, moxibustion group B and naphtha group have significant differences (P<0.05);naphtha group A compared naphtha group B with no significant difference (p>0.05); moxibustion group B, naphtha group A and naphtha group B all have no significant difference (p>0.05).The mice of resist to fatigue test analysis:Comparing with all groups before treatment, there was no significant difference(p>0.05). Comparing with the control group after treatment, naphtha group and moxibustion group all have significant differences (p<0.05); moxibustion group A compared with moxibustion group B have significant differences (p<0.05); naphtha group A compared naphtha group B have no significant difference (p>0.05); moxibustion group A compared with naphtha group A have significant difference (p<0.05);moxibustion group B compared with naphtha group B have significant difference (p<0.05).Comprehensive clinical observation:three groups of participants in age and sex showed no significant difference(p>0.05), suggesting that the general situation of the three groups were comparable. There was no significant difference among the three groups before moxibustion (p>0.05).There was significant difference between 1-year of Ai and 3-year of Ai at the point of 4 and 8(p<0.05).There was significant difference between 1-year of Ai and 5-year of Ai at any point (p<0.05). The difference in temperature between 3-year of Ai and 5-year of Ai was significant(p<0.05).The differences in comfort standards among the three groups were significant(p<0.05). There was no significant difference in comfort standards between 1-year of Ai and 3-year of Ai while there was significant difference between 1-year of Ai and 5-year of Ai. Compared with 3-year Ai,5-year Ai had significant difference (p<0.05). There was no significant difference in acceptance between 1-year of Ai and 3-year of Ai(p>0.05). There was significant difference between 1-year of Ai and 5-year of Ai(p<0.05). Compared with 3-year of Ai,5-year of Ai had significant difference.Conclusion:1.Analysis about essential oil of Different years moxa:the chemical composition was similar between 1-year of Ai and 5-year of Ai, but the contents were different。The flammable fraction in 1-year of Ai was more than that in 5-year of Ai. The involatile fractions were more in 5-year of Ai, and these fractions may be the valid element.2. The mice of analgesia experiments analysis:Moxibustion group and control group were significantly higher volatile caused glacial acetic acid in mice pain threshold, but compared with the positive group,the effect was a bit poor; moxibustion group B was better than moxibustion group A to ascend the pain threshold of mice, volatile oils group B was better than volatile group A in ascending the pain threshold of mice. It suggested that the contents of involatile fractions may be the cause of ascending the pain threshold of mice.3. The mice of resist to fatigue test analysis:Comparing with the control group, naphtha group and moxibustion group all had significant differences, and suggested naphtha and moxibustion could make tired mice get recovery rapidly; moxibustion group B compared with moxibustion group A had significant difference (p<0.05). It can be concluded that 5-year of Ai had fewer flammable fractions than 1-year of Ai, so the penetration 5-year of Ai was better than 1-year of Ai.4. Clinical experiment:The burning speed of 1-year of Ai was the fastest of the three groups.1-year of Ai can raised the temperature of the point fastest. The comfort standards and acceptance standards of 5-year of Ai were higher than 1-year of Ai, and higher than 3-year of Ai, too. The volatile oil of 5-year of Ai was scarce, and the moxa was rich, so the temperature of 5-year of Ai raised gently, and the score of comfort standards was the highest; and maybe the involatile fractions resulted in the highest comfort standards of the subjucts. |
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