| Objectives:1) to observe the possibility and extent of different human lung cancer strains in uptaking 2-18F-ethanol in vitro.2) To study the biological distribution of 2-18F-ethanol in nude mice model of lung cancer plus inflammation by comparing with 18F-FDG in vivo and micro PET imaging.3) to investigate the possibility of 2-18F-ethanol, as a potential PET tracer, in evaluating the effect of chemotherapy in nude mice model of lung cancer.Methods:1) four different lung cancer strains (A549,GLC-82,95C,95D) were incubated by 2-18F-ethanol. The radiation activity of those lung cancer strains were measured 15min,30min,60min,90min,120min and 150min later after the 2-18F-ethanol incubation. The results were analyzed by comparing with 18F-FDG 2) Nude mice model of lung cancer+inflammation were established.25 of them were divided into 5 equal groups of A, B, C, D and E randomly.2-18F-ethanol were injected into mice model in group A, B, C, D, while 18F-FDG were used in group E. At different time point after drug injection (A 30min; B 60min; C 90min; D 120 min; E 60min), radiation activity in each group were measure and illustrated by micro PET imaging.3) Nude mice model of lung cancer were established and 10 of them were divided randomly into chemotherapy group (cisplatin) and non-chemotherapy group (saline). After injection of different drugs for the two groups for 48 hours, the proliferating cell nuclear antigen (PCNA) was tested. Then 2-18F-ethanol was injected into each mouse.90 min later, radiation activities were tested and micro PET imaging were established for nude mice model in each group with comparison.Results:1) all those lung cancer strains (A549,GLC-82,95C,95D) uptook 2-18F-ethanol obviously with peak values of radiation activity of 430.4±25.5, 551.2±16.,597.2±18.8 and 672.7±16.6 after 2-18F-ethanol incubation for 90 minutes separately. The more malignant of lung cancer strains, the higher of the peak values (p<0.05).2) the radiation activity percentage per gram of lung cancer tissue in nude mice model of lung cancer+inflammation was always the highest with a peak value of 7.527±0.34 after 90 minute, despite of various distribution of 2-18F-ethanol in normal tissue and inflammation tissue. Comparing to inflammation tissue, tumor presented statistically higher radiation activity (p<0.05, 7.527±0.34 vs.1.891±0.11). As for normal tissue, higher radiation activities were found mostly in the bone, liver and myocardium, following by lung, brain, spleen and skeletal muscle. The mean 2-18F-ethanol T/NT of tumor vs. normal tissue in the nude mice model was as high as 5.58±1.686 (2.9-9.0), close to that of 18F-FDG (p=0.490>0.05). Under the 2-18F-ethanol micro PET, the tumor presented clear imaging, while the inflammation tissue did not.3) Forty eight hours later, after injection of cisplatin for the chemotherapy group, the PCNA was obviously decreased, comparing the non-chemotherapy group after the same duration but injected only saline (p<0.05). After the injection of 2-18F-ethanol, the mean radiation activity percentage per gram in the chemotherapy group was only 2.513±0.185%/g, statistically lower than 7.366±0.401%/g of non-chemotherapy group (p<0.05). Under micro PET observation, the tumor tissue in the chemotherapy group presented very vague image, comparing to the non-chemotherapy group.Conclusions:2-18F-ethanol can be uptaken by various human lung cancer strains in vitro. In the nude mice model of lung cancer+inflammation, there is more distribution of 2-18F-ethanol in lung cancer tissue than in inflammation tissue and the differences can be distinguished by micro PET.2-18F-ethanol PET may be used in the differential diagnosis of lung cancer. Further, it may be useful in the evaluation of the chemotherapy effect in lung cancer. |
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