Studies On The Warmly Invigorate Kidney-Yang Effect Echanism Of Semen Allii Tuberosi Before And After Stir-heating With Salt Solution

Semen Allii Tuberosi is the dry ripe seed of Alliium tuberosum Rottl, pungent and sweet in flavour, warm, acting on the liver channel and the kidney channel. Its function is warming and tonifing the liver and kidney, invigorating Yang and control nocturnal emission, usually being used to treat impotence and seminal emission, ache of waist and knees, enuresis, frequent micturition, cloudy urine, morbid leucorrhea drolling and emissions, premature ejaculation. There are two kinds of processed forms in'Pharmacopoeia of the People's Republic of China', which are'The Crude Seed Allii Tuberosi (CSAT)'and'The Stir-heated Seed Allii Tuberosi (SSAT)', CSAT is processed by excluding impurity, and SSAT is stir-heating the CSAT with salt solution.ObjectiveThis paper selects Semen Allii Tuberosi as the research object, bases on the background of large differences in its processed ways in history, takes the different processed products'effect of Warmly Invigorate Kidney-Yang as the breakthrough point, intends to preliminary clarify SSAT mechanism and material basis of the Warmly Invigorate Kidney-Yang effect, further interpreting the scientific connotation of the theory for "The stir-heated drug with salt solution acts on kidney", study the stir-heated drug with salt procesed of Semen Allii Tuberosi, and study the product quality standard of SSAT.MethodsThe methods used in the research of the scientific connotation of the theory for the stir-heated CSAT with salt solution are philological, pharmacological and chemical means.1. Systemically arrange the documents about the processing methods of the past and follow up the modern report timely by philological means in order to recognize the history and actuality of CSAT.2. Using pharmacological methods, conduct CSAT four processed products effect of Warmly Invigorate Kidney-Yang pharmacodynamics contrast studing, and also discuss the possible mechanism, recognize the CSAT effect of Warmly Invigorate Kidney-Yang.3. Warmly Invigorate Kidney-Yang mechanism research:using Kidney-Yang deficiency mouse model as the study object, the mechanism was investigated through the mouse mating reproductive organs weight testicular tissue observations; And the determination in plasma corticotropin hormone levels and serum testosterone levels.4. Take efficacy as the guidance by using chemical method, the active fraction with Warmly Invigorate Kidney-Yang effect of SSAT was screened, and the effectively extract quality was be controlled by fingerprint technology.5. On the basis of the study, study stir-baking with salt solution process of CSAT and preliminary study the quality standard of SSAT.Results1. CSAT has function of lowering vitamin C content in adrenaline of Kidney-Yang deficiency mice, and extending time of death of Kidney-Yang deficiency mice in hardy. CSAT through stir-heating with salt solution the function in lowering content of vitamin C in adrenal get further strengthen, with 100 times adult dosage, CSAT play the action, and SSAT play the action with 60 times adult dosage, and the salt played a synergistic role. CSAT through stir-heating with wine solution the function in extending time of death of Kidney-Yang deficiency mice in hardy get further strengthen, with 140 times adult dosage, CSAT play the action, and the product of stir-heating the CSAT with wine solution play the action with 80 times adult dosage, and the rice wine also played a synergistic role.2. CSAT and SSAT could also significantly increase the plasma ACTH and serum testosterone concentration of Kidney-Yang deficiency mice, could also increase mating ability of Kidney-Yang deficiency mice, among them SSAT has better action than CSAT in increasing the plasma ACTH concentration. CSAT and SSAT could also significantly increase the weight of testicle of Kidney-Yang deficiency rat, and could improve the number of sperm cells at birth, among them SSAT has better action than CSAT in increasing the number of primary pure mother cells-subprime pure mother cells and sperm cells.3. "Alcohol water double extract-n-butanol extract+salt" is active fraction with Warmly Invigorate Kidney-Yang effect of SSAT; Fingerprint of active fraction was established by HPLC technology.4. Preliminary established process of stir-baking with salt solution for CSAT is: Every 100kg crude drugs add 2kg salt and 55kg water, moisten in the room temperature for 6 hours and then stir-fry at 150℃with 40 times/min of stirring frequency for 15 minutes.ConclusionsCSAT has certain effect of Warmly Invigorate Kidney-Yang, different processing methods of herbs have influence to their efficacy, stir-baking with salt solution can further enhance the adrenocortical function, stir-baking with wine solution can further improve chillness-tolerance ability, stir-frying solution can benefit crude drugs crushing and promote medicinal ingredients dissolving, the scientific rationality the ancients ways which stir-frying solution for the CSAT and "wine paste for pills and salt soup send service" was proved, and also found the scientific basis for SSAT used in modern. The salt and rice wine both played a synergistic role in effect of Warmly Invigorate Kidney-Yang at different parts. "Alcohol water double extract-n-butanol extract+salt" is active fraction with Warmly Invigorate Kidney-Yang effect of SSAT. The mechanism with Warmly Invigorate Kidney-Yang effect of SSAT lies in increasing corticotropin hormone secretion to enhance adrenocortical function, and its improving the mating ability, testicular weight and various raw number of all kinds of sperm cells may be related with increasing testosterone hormone secretion.Innovations1. Preliminary clarified the influence to Warmly Invigorate Kidney-Yang effect of CSAT by three kinds of processing method which are stir-baking with salt solution stir-baking with wine solution and stir-frying solution.2. Elucidating the synergistic actions of CSAT and salt or rice wine for Warmly Invigorate Kidney-Yang effect, such can certify the TCM theory about "the stir-heated drug with salt solution acts on kidney"3. Elucidating the active components and mechanisms for Warmly Invigorate Kidney-Yang effect of SSAT.