| Objective:Transfer was one of the biological characteristics of malignant tumors, was caused by malignant tumors main causes of death. It was estimated that the incidence trend of 2015, the incidence of cancer worldwide will reach 15 million the number of death to more than 1,000 million. Therefore, how to control tumor metastasis, to a great extent determine the success or failure of cancer therapy was the current basic and clinical research in cancer problem. At present the efficacy of Western medicine was not for the exact tumor metastasis, and in recent years, Chinese medicine in the treatment of cancer efficacy of the transfer of more and more attention. Instructor Professor Qiu Xingfan combined with years of clinical experience that cancer metastasis was a complex interaction of multiple factors, process in which righteousness deficiency, cancer drugs and collaterals flaming Blockage factors leading to tumor metastasis, it was proposed righting Tongluo Toxin control the treatment of tumor metastasis law. Clinical use of that control metastasis of this method can effectively improve the quality of life and prolong survival and reduce mortality. Urban and rural residents in 2006 survey of the major causes of death, lung cancer was the leading cause of death of one of common malignant tumors. The purpose of this subject in the study based on the theory of cancer metastasis to lung cancer, for example, the use of lungs Tongluo Jiedu metastasis of Lewis lung cancer mouse model of spontaneous lung metastasis in experimental anti-cancer study lungs meridians detoxification side Effect and mechanism for the anti-cancer drug development and application of the transfer provide a theoretical basis and experimental evidence.Method:1.The theoretical study, literature review, clinical practice, discusses the etiology and pathogenesis of tumor metastasis and treatment rules.2.Lungs Tongluo Jiedu metastasis of Lewis lung cancer mouse model of spontaneous anti-cancer therapeutic effect and mechanism of transfer of experimental research:The way of animal experiments, the establishment of five groups: normal control group, model control group, lungs Tongluo Jiedu low dose group, lungs meridians Jiedu high dose group, cyclophosphamide group. Through the recovery, vaccination, conservation Lewis lung tumor lines, select a good growth of Lewis lung cancer, cut and homogenized into cells were inoculated in 40 C57BL/6 mice subcutaneously in the right axilla, according to documents copied C57BL/6 spontaneous lung metastasis in mice Lewis lung cancer model. Model successfully reproduced in the model control group, lungs meridians Jiedu low dose group, lungs meridians Jiedu high dose group, cyclophosphamide group began the day after inoculation were given medicines orally, the normal group were given distilled water gavage for 28 days, the first 29 days mice were sacrificed by cervical dislocation. To take samples, make the following test:the normal control group, model control group, lungs meridians Jiedu low dose group, lungs meridians Jiedu high dose group, tumor inhibition rate of cyclophosphamide, anti-inhibition of lung metastasis, spleen index, content of peripheral blood T lymphocyte subsets; RT-PCR method to observe the model control group, lungs meridians toxic side low dose group, lungs meridians Jiedu high dose group, cyclophosphamide group VEGF mRNA tumor cells in mice, nm23 mRNA expression situation; by immunohistochemical detection model control group, lungs meridians toxic side low dose group, lungs meridians Jiedu high dose cyclophosphamide VEGF protein in tumor cells of mice, nm23 protein and AQP-1 protein expression in the situation.Results:1.Through the collation of the literature, clinical practice, that "are virtual network paralysis drug knot" was the basic pathogenesis of cancer metastasis, cancer drugs due to a special evil in the human body caused by this toxin from the accumulation of a variety of pathogenic evil body, falling from mutations, and common drugs were fundamentally different from evil, which we call "cancer drug." Pathogenesis of malignant human body as "righteousness deficiency, cancer, poisoning life", and the pathogenesis of tumor metastasis was the "righteousness deficiency, cancer drug flaming, collaterals Blockage":body upright deficiency, cancer, poisoning the students, leading to tumor production; in cancer, due to improper personal photo transfer, or drug use led to deficiency of righteousness over the further damage, while cancer drug relative to the deficiency of the upright growing flaming, venae Blockage, cancer drugs, or attached to the often toxic, and ultimately suppression was not evil, righteousness breakthrough cancer drug binding, scattered in the body, that the transfer occurred. Cancer drug transfer channel was the body's collaterals; cancer drug transfer destination is susceptible organs dirty air loss. "Righting Tongluo detoxification" treatment for controlling tumor metastasis law, righting the basic prevention and treatment of tumor metastasis, replenishing qi and blood of the upright with a strong, strong upright tonic to the root of the organs; detoxification is the key to prevention and treatment of tumor metastasis, often toxic discharge Students must poisoned by the source of cancer, cancer drugs to absolutely eliminate the root of tumor metastasis; pass network was an important part of prevention and treatment of tumor metastasis, tumor elimination meridians of the body in addition to tumors, worms, tick search in addition to the root of tumor metastasis.2.Lungs meridians toxic side low dose rate of 44.62% inhibition of lung metastasis inhibition rate of 77.95%, compared with cyclophosphamide group were significant differences (P<0.01), that model was successfully complement Tongluo Jiedu lung metastasis of tumor inhibition, inhibition of transfer of the toxic side effects lungs meridians than cyclophosphamide. 3.Lungs meridians Jiedu spleen index of low-dose group compared with the cyclophosphamide group were significantly different (P<0.05), the CD4+/CD8+ ratio, and lungs meridians Jiedu high dose group,low dose group and control group no significant difference (P>0.05),lungs meridians Jiedu high dose group compared with the cyclophosphamide group were significantly different (P<0.05), lungs meridians Jiedu low dose group and cyclophosphamide. There was significant group difference (P<0.01),shows good lungs meridians to improve the immune detoxification Fang Youliang role.4.The expression of VEGF immunohistochemistry results showed that lungs Tongluo Jiedu low dose, toxic side lungs meridians can reduce the high dose group VEGF protein expression, lungs meridians Jiedu high dose group, lungs through Contact Jiedu low dose group compared with the control group, there was a significant difference (P<0.01). RT-PCR detected the expression of VEGF mRNA showed that lungs Tongluo Jiedu low dose group, lungs meridians toxic side can reduce the high dose group, VEGF expression, lungs meridians Jiedu high dose group, lungs meridians Jiedu low dose group compared with the control group, there was a significant difference (P<0.01), that can inhibit the detoxification lungs meridians tumor angiogenesis.5.Immunohistochemistry detected nm23 protein expression in tumor cells showed that lungs with low doses of Tongluo Jiedu, lungs meridians Jiedu high doses could increase the expression of nm23 protein, lungs meridians Jiedu low dose, complement pulmonary collaterals Jiedu high dose group compared with the control group, there was a significant difference (P<0.01); RT-PCR detected the expression of nm23 mRNA tumor cells, the results showed that lungs meridians Jiedu low dose, lungs through Contact Jiedu high doses were increased nm23 expression, lungs meridians Jiedu low dose, lungs meridians Jiedu high dose group compared with the control group, there was a significant difference (P<0.01), that detoxification lungs meridians can only increase the expression of nm23 protein, thereby inhibiting lung metastasis.6.Immunohistochemical detection of tumor cells the expression of AQP-1 protein showed that lungs with low doses of Tongluo Jiedu, lungs meridians high doses may reduce the toxic side AQP-1 protein expression, lower lungs meridians Jiedu dose, lungs meridians Jiedu high dose group compared with the control group, there was a significant difference (P<0.01), that can decrease the lungs meridians detoxification AQP-1 expression, thereby inhibiting lung metastasis.Conclusion:Bufei Tongluo Jiedu inhibit the C57BL/6 mice with Lewis lung metastasis model of spontaneous lung metastasis of lung function can increase the spleen index, increased levels of peripheral blood T lymphocyte subsets, with good immune function improved, but can inhibit angiogenesis, but also enhance the metastasis suppressor gene nm23 cell protein and nm23 mRNA expression levels decreased AQP-1 protein expression. Improve immunity, inhibit angiogenesis, inhibit the expression of VEGF protein and mRNA level, AQP-1 protein expression levels and increased expression of nm23 protein and nm23 mRNA levels may be toxic side lungs meridians transfer of part of the anti-tumor mechanism. |
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