The Research Of The Effective Fraction About Antifebrile Action From Bupleurum Chinese-Scutellaria And Its Compatibility Mechanism

The research of the effective fraction about antifebrile action from Bupleurum Chinese-Scutellaria and its compatibility mechanismTCM formula is the main form of clinical medication. The utility of the compound is not composed of the single herb medicine and the chemical composition of the simple addition is the kinds of components and coexistence the result of joint action.Prescriptions research foundation is the modernization of TCM utility of the important research areas, for the material level in formula of scientific principle and reveal to promote the quality of traditional Chinese medicine to raise the level of all have important significance.This subject is based on traditional Chinese medicine pharmacology of scientific theory as the foundation, for the Xiao-Chai-Hu decocting as the research object, select and Bupleurum-scutellaaria baicalensis on efficacy of closely related antipyretic effect as effect indicators, cl on Chai-Qin drugs on chemical fractions and antipyretic effect of compatibility between relationship; in part based on the optimization of compatibility, further exploration of the antipyretic effective component compatibility mechanism.Research 1:The heating methods of mouse modelMethods:the male NIH mouse 25-30g; We raised them for 3-5 days under the room temperature (23℃). On the 4th and the 5th days we will measure every day anus temperature two times, then pick out the continual 2d single body temperature mouse surpasses 38.℃, or two anus temperature fluctuation surpasses 0.4℃which we take the rectal temperature as the average of the basal body temperature.The male NIH mice were assigned into normal group 1, the model group2,10 mice in every group, inject lipopolysaccharide (LPS)/NS solution (110μg/kg,200μg/kg) intraperitoneally to induce fever in mouse. The normal group and the model group were given the same volume saline. The rectal temperature were measured at Omin,30min,60min, 90min,120min,180min,240min,330 min after LPS injection in mouse and each time point temperature differenceΔ(measured rectal temperature-initial rectal temperature),rendering each average temperature change curve.Results:Intraperitoneal injection of200μg/kg dosage's LPS could cause NIH mice with elevated body temperature obviously,,and the heating duration is longer. The experiment should pay attention to the influence of various factors on rectal temperature. Conclusion:Compared with normal group,200μg model group,110μg model group mice anus temperature raise significantly (P< 0.01, P< 0.05) at different time points after the 30min point; Compared with the 200 u g model group, after 60 min 110μg model group at each time point of anus temperature changes significant differences (P<0.05).Research 2:Exploration of the antipyretic effective fraction from herbal pair of Bupleurum chinese and Scutellaria baicalensis (a) Water extraction and combined alcohol precipitation separation of the decoction Bupleurum chinese and Scutellaria baicalensis herbal pair. Experiment 1:The chai-qin decoction and combined separation on mice induced by LPSΔT heating effect.We used this method to extract the decoction divided into A, B, C, D four chemistry part, determine the separation process of Bupleurum-scutellaaria baicalensis on Antipyretic active ingredients without loss through the observation antipyretic effect on the fever model mice induced by LPS.Experiment 2:The chai-qin parts-Study on the relationship between compatibility and antipyretic action.We combined the separate position according to orthogonal table L8(2)7, The site group and CD interactive group were can significantly reduce the heating mouse rectal temperature (p<0.01). Conclusion:each part and CD interactive group had different degree of antipyretic effect(p<0.01), there existed complicated interactive relations between various parts. (b) Study on antifebrile parts for Chai-qin decoction by using macroporous resin column to purify the decoction of Bupleurum Chinese and Scutellaria baicalensis.Experiment 1:Preliminary Separation of the decoction of Bupleurum Chinese and Scutellaria baicalensis by using macroporous resin column.The four fractions, A (polysaccharides), B(saponins), C(saponins and flavonoids), D (flavonoids and aglycones of saponins), purified by AB-8 macroporous resin, all can reduce the temperature of fever mice (p<0.05), and diferent fraction have differen levels. Fraction D showed the best effective on antifebrile action (p<0.01).Conclusion:The decoction of Bupleurum Chinese and Scutellaria baicalensis involved more than one effect of the fever effective fractions.Experiment 2:Study on the effective parts compatibility and antipyretic effect relationship about the fractions of chai-qin.We combined the separate position according to orthogonal table L8(2)7 method of the research in the second experiment of Research 2; Results:in Chai Qin parts compatibility group to Chai Qin group cd, group abcd, Chai Qin group abd antipyretic effect is better, the best part ofChai Qin compatibility were for the CD group. Conclution:The chai-qin water extracted by macroporous adsorption resin column for the separation of the parts and it's the compatibility were antipyretic effect, part cd was the main utility base.Research 3:The bupleurum chinensis-Huang antifebrile parts compatibility mechanism of action of investigation.We Selected parts of ab, abd, cd, abcd compatibility from Scutellaria baicalensis Radix Bupleuri-water extracted by macroporous adsorption resin column separation, observed Correlation index on the effect of heating model. Results:(1)Compared with the normal group, model group of TNF-α,β-EP content in peripheral serum, TNF-α,PGE2, cAMP, AVP content in hypothalamus, were significantly elevated (P<0.05, P<0.01); (2) Compared with the model group, Chai-Qin cd group and Chai-Qin abed group can obviously reduce the peripheral blood levels of TNF-α,β-EP content (P<0.05), and decreased in hypothalamus of TNF-α,PGE2,cAMP content (P<0.05); Chai-Qin abcd group of hypothalamic PGE2 content were significantly lower (P<0.01), Chai-Qin abd group of hypothalamic cAMP content decreased significantly (P<0.01):on Chai-Qin compatibility group hypothalamic AVP content have decreased trend (P>0.05).Conclusion:The experimental exploration of LPS induction heating mechanism may involve the central medium (PGE2, cAMP, AVP), endogenous pyrogen TNF-α, brain gut peptide (β-EP) of a number of links,Chai-qin antipyretic mechanism of action of the various effective fraction of the compatibility were mainly regulate quenched and tempered PGE2, cAMP of body temperature central,restrain of endogenous pyrogen TNF-α,brain-gut peptideβ-EP synthesis or release to achieve antipyretic effect. But in different parts antipyretic action link is the difference. Antipyretic effect size was related to the antipyretic effect of links number.