| Background:Gastric cancer is the most common malignancy in the world, theincidence ranking second after lung cancer,and mortality rate is very high.Treatment of gastric cancer is mainly to surgical resection.But the survival ratedifferences in general hospitals from30%to50%.Now researches about gastriccancer have become a hot point because of its high incidence mortality andpoor postoperative survival rate. The development of gastric cancer is morethan one factor, but a complex process of gene action.A variety of biologicalmolecules and biological signal transduction pathways are likely to act on theoccurrence of gastric cancer. And different types of gastric cancer relatedmolecules involved in tumor genes too. In recent years there have beenimmune biological therapy, surgical intervention and comprehensive treatmentof postoperative adjuvant chemotherapy.The concept of tissue microarray was introduced by Battifora in thestudying lots of tumor wax blocks.In1998, Konoen defined them as the tissuemicroarray(TMA). A slice of TMA can accommodate0.6-2.0mm donerorganization about400-1000. Kononen used tissue microarray studying ongene expression in breast cancer, confirming the utility of this technology, anddeclared the birth of the concept of tissue microarray. And soon the applicationof tissue microarray bloomed in the life sciences of basic research and theclinical areas.The principle of TMA draw from ideology of the parallelanalysis.A robotic arm would be used to transfer thousands of tissue into thefixed wax block.TMA is a high-throughput detection technology.TMA has the characters of small size and containing lots of informations.Compareing withtraditional methods,TMA is just one glass slide and the experimental conditionsis to be maximum extently consistent,highly accurate,comparabile.In the earlier period,Dr Wang applied Protein Pathway array technology tofind nine kinds of gastric cancer-associated protein upregulated in gastriccancer patients.To furtherly validate the results,we selected these proteins(CDK2,XIAP,CDK6,AKT,NOTCH4,PCNA,β-CATENIN,p-PKCα,p-PKCα/β2).We made the TMA containing126gastric cancers and30normalgastric tissues.By immunohistochemistry we observe the results with thedegree of tumor differentiation,gastric cancer staging,and prognosis.So that itcan provide a theoretical basis for further study on gastric cancer.Materials and Methods:Specimens collection:Specimens came from126patients with gastriccancer undergoing surgery in First Hospital of Jilin University from year of2000to2006.All patients had a complete clinical data and postoperativevisiting information.Another concomitant30cases of normal gastric tissue gotas the control team.All specimens were through10%formalin-fixed, conven-tional paraffin embedding.Methods:We collected156paraffin tissue and TMA was constructed(5cases of gastric carcinoma and1case of normal tissue off-chip).Afterimmunohistochemical staining,two pathologists independently read the slidesto determine the results.We used SSPS19.0software to analyzing theresults:the application of chi-square test to discriminate the difference betweenthe two groups;the spearman correlation test used;Kaplan-meier method on thesurvival time of patients.Cox regression analysis on affecting the factors ofsurvival time of patients.Results:1.Nine kinds of gastric cancer-related protein in gastric cancer and normal issue comparision,their P-value less than0.05,considered statist-icallysignificant.That is to say the proteins are highly expressed in gastric cancertissues.2.The analysis of nine kinds of gastric cancer-associated proteins inpatients with age,gender,pathological type,depth of tumor invasion,number oflymph node metastasis and TNM staging showed that:AKT1affects the depthof tumor invasion;NOTCH4affects the pathological type of gastric cancerpatients;β-CATENIN affects the number of lymph node metastasis ofpatients;CDK2affects the depth of invasion and the clinical staging.3.Kaplan-meier method for survival analysis of nine proteins shows:thefive-year survival rate of AKT1staining strongly positive and CDK2positivepatients significantly reduced.4.The nine kinds proteins into the model constructed by the clinicalparameters shows that:AKT1and CDK2is relatively independent factors ofsurvival limition.Conclusion:1.The nine kinds of protein of gastric cancer were elevated.Gastric canceris the results of a variety of intermolecular interaction.And we provide atheoretical basis of pathological diagnosis with gastric cancer patients.2.AKT1and CDK2expression in gastric cancer patients will reduce the5-yuar survival rate.So that they are relatively independent factors affectingprognosis. |
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