Inhibitory Effects Of Paclitaxel-loaded Ultrafine Biodegradable Fibers Again Mice Cervical Cancers

Purpose:To examine the ability of the paclitaxel (PTX)-loaded polylactide fibermats to induce cell apoptosis of U14cervical cancer cell line and to inhibit thegrowth of the autograft U14cervical cancer.Methods:1,The cytotoxicity experiment about nanofiber polymer:in vitro methods。2,U14cervical cancer cell were incubated for48h in the presence of thefiber mats and the cell apoptosis rate (CAR) was evaluated by flow cytometry(FC) with Annexin V-FITC and PI double staining.3,U14cervical cancer models were established in balb-c mice.Studyingthe contribution of biodegradable nanofiber polymer administering taxol inlocal chemotherapy the the balb-c mice cervical cancer on the basis of themodel。4,The37tumor-bearing mice (tumor volume100250mm~3) were dividedrandomly into three groups. group A: control group without any treatments;group B: Fiber-only group;group C: Fiber-PTX group. Under the condition ofanaesthesia, incisions of arc shape were made on the tumors of groups B and Cafter local disinfection. The tumor body was separated as much as possiblefrom the surrounding tissues under the precondition of not destroying the mainblood supplies. The isolated tumor body was wrapped with nano-fiber mats in acoverage ratio of7075%. Then the skin was closed and the incision wassewed. The fiber mats used in group B were electrospun polylactide fiberswithout drug loading while group C fibers contained33wt%of PTX. Seven andfourteen days after surgery,57animals in each group were sacrificed and the tumors were separated, cut down, photographed, and weighed. The tumorinhibition rates (TIR) were calculated.Results:(1)Paclitaxel/PEG-PLA electrospun fiber mats were successfully preparedby electrospinning.(2)Cell's counting method, MTT method confirmation the biodegradablenanofiber polymer administered taxol exist time, dosage dependence on repressthe U14cells.(3)The FC results showed that after48h incubation in the presence ofPTX-loaded fibers (equivalent PTX content40μg/ml), the alive cells were72.5%, the early apoptotic cells were20.5%, the late apoptotic cells were5.1%.The total CAR (25.6%) was significantly higher than the Control group (1.0%)and the Fiber-only group (1.5%).(4)The PTX-loaded fiber wrapping led to significant inhibition of thegrowth of U14cervical cancers. The TIR with respect to the Control group was47.8%on the7th day and56.0%on the14th day (P<0.05). Although the groupB animals underwent surgery and their tumors were wrapped with the fibermats without drug loading, their tumor weights on the7th day and14th daywere not significantly different from those of the Control group, indicating thatthe polylactide fibers themselves did not inhibit the tumor growth and theinhibitory effects observed in the Fiber-PTX group was due to the releasedPTX from the fibers.Conclusion:1,The biodegradable nanofiber polymer administer taxol exist time,dosage dependence on repress the U14cells.2,The biodegradable nanofiber polymers administer taxol to fasten theU14cell induced the apoptosis function.3,The established stereotactic balb-c mice U14cervical cancer models. It is more stable and credibile.4,The PTX-loaded nano-fiber mats displayed significant inhibition to thetumor growth of autograft U14cervical cancer. They are suitable for localchemotherapy of the U14cervical cancer.Creative feature:1.To examine the ability of the paclitaxel (PTX)-loaded polylactide fibermats to induce cell apoptosis of U14cervical cancer cell line and to inhibit thegrowth of the autograft U14cervical cancer.2. The fiber mats used in group C are electrospun polylactide fiberscontaining33wt%of PTX.3.The PTX-loaded nano-fiber mats display significant inhibition to thetumor growth of autograft U14cervical cancer. They are suitable for localchemotherapy of the U14cervical cancer.