| ObjectiveMalignant tumors have become a major kind of diseases which hazard to human health, chemotherapy has now become an important means of the comprehensive treatment in clinical oncology. Chemotherapy can kill and wound residual tumor cells in vivo, enhance the curative effect and improve the prognosis. But the side effect of chemotherapy (bone marrow suppression) is the main cause which makes patients unable to sufficiently complete chemotherapy on schedule, or makes patients die for serious infection. To bone marrow suppression, at present, western medicine hasn't effective prevention and treatment ways, for this reason, it has important clinical significance to probe into traditional chinese medicine treatment which can prevent bone marrow suppression after malignant tumor chemotherapy. This topic is based on the theory of "the kidney stores essence, and the essence can grow into marrow, then the marrow produces blood".According to the previous studies, Morinda officinalis How polysaccharide and stilbene glucoside were selected as the representative of tonify the Kidney, they were respectively from the Morinda officinalis How and Polygonum multiforum Thunb.Then the effect which these two ingredients act on bone marrow suppression was observed, then the mechanism of the effect was studied from the perspective of marrow stem cells. It provides science experimental evidence for using combination traditional Chinese and western medicine to treat the bone marrow suppression after chemotherapy in various malignant tumorMethods1,《Chinese Medical Classics》,《The Integration Of Ancient And Modern Medical Books All The Record》 etc search tools was applicated to systematically analyze the theory of "the kidney stores essence", for seeking the commonality between the function of "the kidney stores essence" in Traditional Chinese Medicine theory and the function of bone marrow stem cell in western medicine, also for providing science theoretical evidence for the bone marrow suppression recovery of using traditional Chinese medicine in malignant tumor after chemotherapy.2,Experiment study(1) Animal Model Making:According to the Lena A Basile method etc,the cyclophosphamide (250mg/kg) was used for intraperitoneal injection and then the model was successfully made at one-time.(2) Grouping:Experimental mice were divided into four groups, they were pretreatment group,treatment group,bone marrow suppression model group, blank control group, each group containing12.(3) dosing:Preconditioning group were gavaged during the lst-7th day, treatment group were gavaged at the8th day, both team were gavaged once a day with the aqueous solution of Morinda officinalis How polysaccharide or THSG. The cyclophosphamide (250mg/kg) was used for intraperitoneal injection in preconditioning group,treatment group,bone marrow suppression model group. Blank control group were gavaged once a day with the same amount of saline.(4) indicator testing:①blood routine:The blood were took from preconditioning group when the1st,8th,12th,15th day and other group when the8th,12th,15th day.②cell count:Using the microscope observed nucleated bone marrow cells,then counted.③The expression of positive C-kit and CD105of bone marrow stem cells were tested by flow cytometry④GM-CSF,SCF/MGF were tested by ELESA method.Results1,Morinda officinalis How polysaccharide(1)The effect on regulation of blood composition of bone marrow suppression model group:The content of red blood cell in bone marrow suppression model group was significantly lower than it in blank control group after chemotherapy at the7th day, it means that red blood cell had been reached low point.But it had no significantly difference between treatment group and blank control group, it showed that Morinda officinalis How polysaccharide could slow down the decline of the RBC in bone marrow suppression model group after chemotherapy. It also had been found that the HGB in bone marrow suppression model group was significantly different from blank control group at the12th day, but it had no significantly difference between pretreatment group and blank control group.(2) The effect on proliferation of bone marrow stem cell in the bone marrow suppression model:After chemotherapy4days, the value of positive CD117in pretreatment group was significantly higher than it in treatment group(P<0.05), while CD105-CD117Double pretreatment group was higher than bone marrow suppression model group (P<0.05). Getting specimen after chemotherapy4days, the bone marrow cells proportion of G0/G1decreased significantly in pretreatment group, treatment group and bone marrow suppression model group compared with blank control group. The proportion of bone marrow cells in phase S and G2/M increased significantly than bone marrow suppression model group, while the proportion of G0/G1in pretreatment group decreased significantly and increased significantly in phase S compared with treatment group.(3) The effect on secretion of stem cell growth factor:The concentration of GM-CSF in pretreatment group was significantly higher than bone marrow suppression model group, the difference was significant (P<0.05)2,Stilbene Glucoside(1) The effect on regulation of blood composition of bone marrow suppression model group:All groups descended significantly except blank control group, at the4th day after chemotherapy, but the value of white blood cells of pretreatment group were significantly higher than it in treatment group and marrow suppression model group (P<0.05). The value of RBC and HGB significantly descended than blank control group (P<0.01), but it had no significant difference between pretreatment group and blank control group.(2) The effect on proliferation of bone marrow stem cell in the bone marrow suppression model:the value of positive CD117in pretreatment group and treatment group was significant higher than it in blank control group (P<0.05), at the4th day after chemotherapy;The value of positive CD105increased in all groups compared with blank control group, while the value of treatment group was significant higher than pretreatment group's (P<0.05); Getting specimen after chemotherapy4days, the bone marrow cells proportion of G0/G1descended significantly in pretreatment group, treatment group and bone marrow suppression model group compared with blank control group.The proportion of bone marrow cells in phase S and G2/M significantly increased than bone marrow suppression model group, while the proportion of G0/G1in pretreatment group decreased significantly and increased significantly in phase S compared with treatment group, and then returned to normal level after7days.(3) The effect on secretion of stem cell growth factor:The concentration of SCF/MGF in pretreatment group was significantly different from blank control group, bone marrow suppression model group and treatment group (P<0.05)Conclusion:1.Morinda officinalis how polysaccharide has the promoting effect on mice which had been in bone marrow suppression after chemotherapy. It increases the proportion of S and the G2/M phase bone marrow stem cells, enhances the number of stem cells in bone marrow, promotes the secretion of G-CSF, and increases red blood cells and hemoglobin of the peripheral blood cells, and then improves the toxic and side effects of bone marrow suppression after chemotherapy.2. Stilbene Glucoside has the promoting effect on mice which has been in bone marrow suppression after chemotherapy, it also increases the expression of positive CD117of bone marrow stem cells, the secretion of G-CSF, and the white blood cells of the peripheral blood cells, and then improves the toxic and side effects of bone marrow suppression after chemotherapy. Using Stilbene Glucoside after chemotherapy can increase the expression of positive CD117and CD105of bone marrow stem cells, promote the proliferation of bone marrow stem cells, and then increase the red blood cells,hemoglobin. It has positive regulation function to bone marrow suppression after chemotherapy after chemotherapy. |
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